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Titolo:
TARGETING OF AN INTESTINAL APICAL ENDOSOMAL PROTEIN TO ENDOSOMES IN NONPOLARIZED CELLS
Autore:
WILSON JM; COLTON TL;
Indirizzi:
UNIV ARIZONA,COLL MED,DEPT CELL BIOL & ANAT,STEELE MEM CHILDRENS RES CTR,POB 245044 TUCSON AZ 85724 UNIV ARIZONA,STEELE MEM CHILDRENS RES CTR,DEPT PEDIAT TUCSON AZ 85724
Titolo Testata:
The Journal of cell biology
fascicolo: 2, volume: 136, anno: 1997,
pagine: 319 - 330
SICI:
0021-9525(1997)136:2<319:TOAIAE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUCKLING RAT ILEUM; MANNOSE 6-PHOSPHATE RECEPTORS; SYNAPTIC VESICLE PROTEINS; POLARIZED CACO-2 CELLS; COATED PIT FORMATION; TRANS-GOLGI NETWORK; BREFELDIN-A; ENDOCYTIC PATHWAYS; EPITHELIAL-CELLS; ABSORPTIVE CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
71
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Wilson e T.L. Colton, "TARGETING OF AN INTESTINAL APICAL ENDOSOMAL PROTEIN TO ENDOSOMES IN NONPOLARIZED CELLS", The Journal of cell biology, 136(2), 1997, pp. 319-330

Abstract

Polarized cells such as epithelial cells and neurons have distinct endosomal compartments associated with different plasma membrane domains. The endosomes of the neuronal cell body and the basolateral cytoplasm of epithelial cells are thought to perform cellular ''housekeeping''functions such as the uptake of nutrients and metabolites, while the endosomes in the apical cytoplasm or axons are thought to be specialized for the sorting and transcytosis of cell type-specific ligands and receptors. However, it is not known if nonpolarized cells such as fibroblasts contain a specialized endosomal compartment analogous to the specialized endosomes found in neurons and epithelia. We have expresseda protein that is normally found in the apical early endosomes of developing intestinal epithelial cells in normal rat kidney fibroblasts. This apical endosomal marker, called endotubin, is targeted to early endosomes in transfected fibroblasts, and is present in peripheral as well as perinuclear endosomes. The peripheral endosomes that contain endotubin appear to exclude transferrin, fluid phase markers, and the mannose-6-phosphate receptor, although in the perinuclear region colocalization of endotubin and these markers is present. In addition, endotubin positive structures do not tubulate in response to brefeldin A andinstead redistribute to a diffuse perinuclear location. Since this endosomal compartment has many of the characteristics of an apical or axonal endosomal compartment, our results indicate that nonpolarized cells also contain a specialized early endosomal compartment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:33:09