Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECT OF SPERM PREPARATION WITH TEST YOLK BUFFER ON SPERM-BINDING CAPACITY UNDER HEMIZONA ASSAY CONDITIONS
Autore:
GAMZU R; YOGEV L; BOTCHAN A; AMIT A; LESSING JB; LICHTENBERG D; PAZ G; YAVETZ H;
Indirizzi:
TEL AVIV UNIV,TEL AVIV SOURASKY MED CTR,SERLIN MATERN HOSP,INST STUDYFERTIL,IVF UNIT,POB 7079 IL-69970 TEL AVIV ISRAEL TEL AVIV UNIV,TEL AVIV SOURASKY MED CTR,SERLIN MATERN HOSP,INST STUDYFERTIL,IVF UNIT IL-69970 TEL AVIV ISRAEL TEL AVIV UNIV,SACKLER SCH MED IL-69978 TEL AVIV ISRAEL TEL AVIV UNIV,SACKLER FAC MED,DEPT PHYSIOL & PHARMACOL IL-69978 TEL AVIV ISRAEL
Titolo Testata:
Andrologia
fascicolo: 1, volume: 29, anno: 1997,
pagine: 17 - 21
SICI:
0303-4569(1997)29:1<17:EOSPWT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-SPERMATOZOA; EGG-YOLK; ZONA-PELLUCIDA; ACROSOME REACTION; IN-VITRO; FERTILIZATION; ABILITY;
Keywords:
SPERMATOZOA; EGG YOLK; HEMIZONA ASSAY; HUMAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
R. Gamzu et al., "EFFECT OF SPERM PREPARATION WITH TEST YOLK BUFFER ON SPERM-BINDING CAPACITY UNDER HEMIZONA ASSAY CONDITIONS", Andrologia, 29(1), 1997, pp. 17-21

Abstract

The study was conducted to evaluate the diverse effect and clinical significance of TEST yolk buffer treatment on sperm samples of 128 infertile men. Sperm samples were incubated with TEST yolk buffer and control medium (Ham's F-10) at room temperature for 2 h. The hemizona indices (mean +/- SE) of the TEST yolk buffer and medium-treated sperm samples were 29+/-2.3% and 22+/-1.6%, respectively. Inspection of the individual response of each sperm sample to TEST yolk buffer revealed that 63 samples (49%) improved (double the interassay variation=28%) their binding to zona pellucida, 36 (28%) remained unchanged, whereas the binding capacity of 29 samples (23%) decreased. Furthermore, TEST yolkbuffer treatment of 24 samples (19%) resulted in an increased bindingbeyond the hemizona index threshold set up at 23%. This level was previously shown to be the cut-off point between fertile and infertile sperm samples. It was concluded that when applied to an unselected groupof infertile men, TEST yolk buffer significantly increased sperm binding capacity to the zona pellucida. However, only 19% of the sperm samples showed improvement with clinical significance. The other sperm samples may have improved, remained unchanged or even deteriorated independently on basic sperm variables. Thus, the effect of TEST yolk buffer treatment on sperm binding should be tested prior to its clinical use to avoid possible damage to certain sperm samples.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 00:19:09