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Titolo:
INFLUENCE OF PLASMIDS ON CATALASE AND SUPEROXIDE-DISMUTASE ACTIVITIESIN LISTERIA-MONOCYTOGENES
Autore:
ISOM LL; AHMED AH; MARTIN SE;
Indirizzi:
UNIV ILLINOIS,DEPT FOOD SCI,569 BEVIER HALL,905 S GOODWIN AVE URBANA IL 61801 UNIV ILLINOIS,DEPT FOOD SCI URBANA IL 61801 ABBOTT LABS ABBOTT PK IL 60064
Titolo Testata:
Journal of food protection
fascicolo: 9, volume: 58, anno: 1995,
pagine: 955 - 959
SICI:
0362-028X(1995)58:9<955:IOPOCA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; RESISTANCE; VIRULENCE; CADMIUM; STRAIN;
Keywords:
LISTERIA; PLASMID CATALASE; SUPEROXIDE DISMUTASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
L.L. Isom et al., "INFLUENCE OF PLASMIDS ON CATALASE AND SUPEROXIDE-DISMUTASE ACTIVITIESIN LISTERIA-MONOCYTOGENES", Journal of food protection, 58(9), 1995, pp. 955-959

Abstract

Two of nine strains of Listeria monocytogenes examined were found to contain plasmid DNA. Strain 19112 contained a 31.1 kb plasmid and strain 7644 contained a 49.4 kb plasmid. Each of the strains was cured of its plasmid by heat treatment at 46 degrees C. Both the wild-type and plasmid-negative forms of each strain were screened for cadmium resistance. The 31.1 kb plasmid of L. monocytogenes 19112 was shown to confer resistance to cadmium. Listeria monocytogenes 7644 did not show resistance to cadmium. The plasmids for both strains were isolated and purified by CsCl density-gradient centrifugation. Each plasmid was electroporated back into the respective plasmid-negative strain. The catalase (CA) and superoxide dismutase (SOD) activities were determined for the wild type, plasmid-negative and electroporated strains. There was asignificant decrease in CA and SOD activities upon loss of the plasmid from each strain of L. monocytogenes. Strain 19112 showed a 36% decrease in CA activity and an 81% decrease in SOD activity as a result ofplasmid removal. Strain 7644 showed a 22% decrease in both CA and SODactivities following plasmid lass. Catalase and SOD activity levels increased for both strains following reinsertion of the plasmid throughelectroporation. Catalase and SOD activity levels of L. monocytogenes7644 were higher for the transformed strain than those of the wild type. Catalase and SOD activity levels of transformed L. monocytogenes 19112 were less than in the corresponding wild type. It appears that plasmids in L. monocytogenes strains 19112 and 7644 may be involved in influencing the regulation of the production of CA and SOD. Plasmid copy number may influence the level of activity of these enzymes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 03:31:53