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Titolo:
LIQUID-CHROMATOGRAPHIC DETERMINATION OF D-AMINO-ACIDS AND L-AMINO-ACIDS BY DERIVATIZATION WITH O-PHTHALDIALDEHYDE AND N-ISOBUTYRYL-L-CYSTEINE - APPLICATIONS WITH REFERENCE TO THE ANALYSIS OF PEPTIDIC ANTIBIOTICS, TOXINS, DRUGS AND PHARMACEUTICALLY USED AMINO-ACIDS
Autore:
BRUCKNER H; WESTHAUSER T; GODEL H;
Indirizzi:
UNIV HOHENHEIM,INST FOOD TECHNOL D-70593 STUTTGART GERMANY HEWLETT PACKARD GMBH,WALDBRONN ANAL DIV D-76337 WALDBRONN GERMANY
Titolo Testata:
Journal of chromatography
fascicolo: 1, volume: 711, anno: 1995,
pagine: 201 - 215
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-COLUMN DERIVATIZATION; AUTOMATED PRECOLUMN DERIVATIZATION; ACETYL-L-CYSTEINE; ORTHO-PHTHALALDEHYDE; CHIRAL THIOLS; FLUORESCENCE DETECTION; FOOD SAMPLES; ENANTIOMERS; RESOLUTION; SEPARATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
H. Bruckner et al., "LIQUID-CHROMATOGRAPHIC DETERMINATION OF D-AMINO-ACIDS AND L-AMINO-ACIDS BY DERIVATIZATION WITH O-PHTHALDIALDEHYDE AND N-ISOBUTYRYL-L-CYSTEINE - APPLICATIONS WITH REFERENCE TO THE ANALYSIS OF PEPTIDIC ANTIBIOTICS, TOXINS, DRUGS AND PHARMACEUTICALLY USED AMINO-ACIDS", Journal of chromatography, 711(1), 1995, pp. 201-215

Abstract

In order to evaluate and extend the applicability of an analytical method that enables the quantitative and simultaneous high-performance liquid chromatographic determination of D- and L-amino acids (DL-AAs) by automated precolumn derivatization with o-phthaldialdehyde together with the chiral thiol N-isobutyryl-L-cysteine [J. Chromatogr, 666 (1994) 259] selected natural and synthetic bioactive peptides, as well as pharmaceutically used formulations of AA, were investigated and the amounts of D- and L-AA determined by fluorescence detection, Peptides containing cyst(e)ine were oxidized with performic acid prior to hydrolysis with 6 M HCl, and those containing Trp were hydrolyzed with 4 M methanesulfonic acid (24 h at 110 degrees C in both cases). Peptides analyzed were the peptide antibiotics bacitracin, gramicidins A and S, polymyxin B, metanicin C, the peptide toxin malformin A and the peptide drugs D-Arg-[Hyp(3),Thi(5,8),D-Phe(7)]-bradykinin, beta-casomorphin and alpha(sl)-exorphin. Further, the enantiomeric ratios of pharmaceutically used AA formulations containing racemic DL-Ser, DL-AsP and DL-Metwere determined, and the AA drugs L-Asp and L-Trp were tested negatively for the presence of the respective D-enantiomers. In two aqueous formulations of L-AA used for parenteral nutrition, low amounts of D-AA(0.1-0.9% with respect to certain L-AA enantiomers and of totally 128mg and 149 mg D-AAs per liter infusion solution) were determined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:17:59