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Titolo:
IMMUNIZATION OF MONKEYS WITH RECOMBINANT COMPLIMENTARY DEOXYRIBONUCLEIC-ACID EXPRESSED ZONA-PELLUCIDA PROTEINS
Autore:
VANDEVOORT CA; SCHWOEBEL ED; DUNBAR BS;
Indirizzi:
BAYLOR COLL MED,DEPT CELL BIOL,1 BAYLOR PLAZA HOUSTON TX 77030 BAYLOR COLL MED,DEPT CELL BIOL HOUSTON TX 77030 UNIV CALIF DAVIS,CALIF REG PRIMATE CTR DAVIS CA 95616
Titolo Testata:
Fertility and sterility
fascicolo: 4, volume: 64, anno: 1995,
pagine: 838 - 847
SICI:
0015-0282(1995)64:4<838:IOMWRC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACACA-FASCICULARIS; HETEROIMMUNIZATION; ANTIBODIES; ANTIGENS; RABBITS; OOCYTE; GENES;
Keywords:
IMMUNOCONTRACEPTION; ZONA PELLUCIDA; FERTILITY; OVARIAN FUNCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Vandevoort et al., "IMMUNIZATION OF MONKEYS WITH RECOMBINANT COMPLIMENTARY DEOXYRIBONUCLEIC-ACID EXPRESSED ZONA-PELLUCIDA PROTEINS", Fertility and sterility, 64(4), 1995, pp. 838-847

Abstract

Objective: To evaluate the effects of immunization with zona pellucida (ZP) proteins produced by recombinant complementary DNA (cDNA) technology for the elicitation and antibodies that inhibit sperm binding without altering ovarian function in the nonhuman primate. Design: Controlled nonhuman primate study. Setting: Controlled environment with individual housing of monkeys in facility approved by National Institutesof Health (NIH) guidelines. Participants: Monkeys housed and treated according to NIH regulations. Interventions: Monkeys immunized and boosted at regular intervals with ZP proteins produced using recombinant cDNA techniques. Main Outcome Measure: Urinary estrogen, P, serum antibody levels, sperm-ZP binding, and ovarian morphology. Results: Monkeys immunized with a recombinant rabbit 75-kd ZP protein expressed from a partial cDNA in the pEX bacteria expression system produce antibodies that interfere with ovarian follicular development and ovarian cyclicity. On the contrary, monkeys immunized with a recombinant rabbit 55-kd ZP protein develop antibodies that inhibit homologous sperm bindingbut do not affect ovarian follicular development or subsequent ovarian hormonal cyclicity. Conclusion: Monkey antibodies to the rabbit 75-kd ZP recombinant protein can be generated that inhibit ovarian cyclicity as desired for animal sterilization vaccines. Antibodies to the 55-kd ZP recombinant protein inhibit homologous monkey sperm binding to the ZP without altering ovarian endocrine function or morphology as is desired for human immunocontraception.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 19:19:52