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Titolo:
EFFECTS OF IONS SUBSTITUTIONS AND OF INHIBITORS ON TRANSEPITHELIAL POTENTIAL DIFFERENCE AND SODIUM FLUXES IN PERFUSED GILLS OF THE CRAB PACHYGRAPSUS-MARMORATUS
Autore:
PIERROT C; PEQUEUX A; THUET P;
Indirizzi:
UNIV MONTPELLIER 2,ECOPHYSIOL INVERTEBRES LAB,PL E BATAILLON,CP 092 F-34095 MONTPELLIER 5 FRANCE UNIV LIEGE,PHYSIOL ANIM LAB B-4020 LIEGE BELGIUM
Titolo Testata:
Archives of physiology and biochemistry
fascicolo: 4, volume: 103, anno: 1995,
pagine: 466 - 475
SICI:
1381-3455(1995)103:4<466:EOISAO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARCINUS-MAENAS; ERIOCHEIR-SINENSIS; CHINESE CRAB; SHORE CRAB; FIDDLER CRAB; UCA-TANGERI; FRESH-WATER; BLUE-CRAB; CL FLUXES; AMILORIDE;
Keywords:
CRAB; GILL; SODIUM FLUXES; TRANSEPITHELIAL POTENTIAL DIFFERENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
C. Pierrot et al., "EFFECTS OF IONS SUBSTITUTIONS AND OF INHIBITORS ON TRANSEPITHELIAL POTENTIAL DIFFERENCE AND SODIUM FLUXES IN PERFUSED GILLS OF THE CRAB PACHYGRAPSUS-MARMORATUS", Archives of physiology and biochemistry, 103(4), 1995, pp. 466-475

Abstract

With the same saline on both sides of the epithelium, a spontaneous inside negative transepithelial potential difference (PD) was measured in perfused posterior gills of the euryhaline crab Pachygrapsus marmoratus acclimated to dilute sea water. The origin of the PD and the transport properties of the epithelium were investigated by ionic substitutions and by application of inhibitors. Diffusion of Na+ and Cl- ions at the apical side contributes to the establishment of the PD. Sodium cyanide (10(-2) M) added to the perfusion and incubation media almost completely inhibited the transepithelial PD and considerably decreasedthe Na+ influx. Internally perfused ouabain (5.10(-3) M) halved the PD and the Na+ influx but had no effect on the Na+ efflux. Externally applied amiloride (10(-3) M) also reduced the Na+ influx by 27%. All inhibitions were concentration-dependent. From these results, it has been concluded that the transepithelial PD and Na+ influx are, at least partly, generated by active, metabolic energy-requiring processes. The effect of ouabain supports the existence of a Na+/K+ exchange mechanism linked to the presence of Na+-K+ ATPase in the basolateral membrane of posterior gills. The effect of amiloride is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 09:04:23