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Titolo:
THE USE OF MICHELS TRANSPORT MEDIUM FOR IMMUNOFLUORESCENCE AND IMMUNOELECTRON MICROSCOPY IN AUTOIMMUNE BULLOUS DISEASES
Autore:
JONES SAV; PALMER I; BHOGAL BS; EADY RAJ; BLACK MM;
Indirizzi:
ST THOMAS HOSP,ST JOHNS INST DERMATOL,DEPT DERMATOPATHOL,LAMBETH PALACE RD LONDON SE1 7EH ENGLAND ST THOMAS HOSP,ST JOHNS INST DERMATOL,DEPT IMMUNOFLUORESCENCE LONDON SE1 7EH ENGLAND ST THOMAS HOSP,ST JOHNS INST DERMATOL,DEPT CELL PATHOL LONDON SE1 7EHENGLAND
Titolo Testata:
Journal of cutaneous pathology
fascicolo: 4, volume: 22, anno: 1995,
pagine: 365 - 370
SICI:
0303-6987(1995)22:4<365:TUOMTM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCALIZATION; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
S.A.V. Jones et al., "THE USE OF MICHELS TRANSPORT MEDIUM FOR IMMUNOFLUORESCENCE AND IMMUNOELECTRON MICROSCOPY IN AUTOIMMUNE BULLOUS DISEASES", Journal of cutaneous pathology, 22(4), 1995, pp. 365-370

Abstract

We report the use of Michel's solution, already a well established transport medium, in the combined use of direct immunofluorescence (IMF)and pre-embedding immunoelectron microscopy in 3 subepidermal bullousdiseases - bullous pemphigoid (BP), epidermolysis bullosa acquisita (EBA) and dermal binding linear IgA disease (LABD). Our studies demonstrated that electron microscopy of normal skin maintained in Michel's medium for up to 28 days showed remarkable preservation of all components of the basement membrane zone, including the ultrastructure of the basal keratinocytes, dermoepidermal junction and papillary dermis. However, epidermal cell cytolysis occurred after just 48 hours. Immunoelectron microscopy using a gold probe has enabled us to localise the immunoreactants in bullous pemphigoid, epidermolysis bullosa acquisita and dermal-binding linear IgA bullous dermatoses. Our findings are comparable to and as equally reliable as those on immunoelectron microscopyof fresh skin biopsies with no loss of antigen deposition, and demonstrate an effective new use of a well established transport medium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 02:28:55