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Titolo:
NEW INSIGHTS INTO THE GATING MECHANISMS OF CARDIAC RYANODINE RECEPTORS REVEALED BY RAPID CHANGES IN LIGAND CONCENTRATION
Autore:
SITSAPESAN R; MONTGOMERY RAP; WILLIAMS AJ;
Indirizzi:
NATL HEART & LUNG INST,DEPT CARDIAC MED,DOVEHOUSE ST LONDON SW3 6LY ENGLAND
Titolo Testata:
Circulation research
fascicolo: 4, volume: 77, anno: 1995,
pagine: 765 - 772
SICI:
0009-7330(1995)77:4<765:NIITGM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-RELEASE CHANNELS; SARCOPLASMIC-RETICULUM; ACTIVATION; MUSCLE; INACTIVATION; CAFFEINE; VESICLES;
Keywords:
RYANODINE RECEPTORS; CA2+-RELEASE CHANNELS; CARDIAC EXCITATION-CONTRACTION COUPLING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
R. Sitsapesan et al., "NEW INSIGHTS INTO THE GATING MECHANISMS OF CARDIAC RYANODINE RECEPTORS REVEALED BY RAPID CHANGES IN LIGAND CONCENTRATION", Circulation research, 77(4), 1995, pp. 765-772

Abstract

We have developed a novel technique for incorporation of sheep cardiac sarcoplasmic reticulum (SR) Ca2+-release channels into planar phospholipid bilayers in order to investigate changes in [Ca2+] on a physiological time scale and have investigated whether the rate of change of cytosolic [Ca2+] has a direct effect on the gating of the cardiac SR Ca2+-release channel. Vesicles of heavy SR were incorporated into planar phospholipid bilayers painted on glass pipettes, and an established technique for rapid solution exchanges at excised membrane patches wasmodified to allow solution changes to be made at the bilayer within 10 ms. For a given change in [Ca2+], we demonstrate that the open probability (Po) is similar whether the cytosolic [Ca2+] is increased rapidly (10 ms) or slowly (1 s) and appears to be no different from the Po measured under steady state conditions that were recorded by using conventional bilayer techniques. We also demonstrate that no desensitization or inactivation occurs at -40 mV when the channel is activated by Ca2+ alone or in the presence of other channel activators, ATP or EMD 41000. However, at +40 mV, rapid channel activation followed by inactivation was observed. The probability of such voltage-dependent inactivation appears to depend on the mechanism of channel activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:23:29