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Titolo:
CALCIUM-INDEPENDENT, MEIOTIC SPINDLE-DEPENDENT METAPHASE-TO-INTERPHASE TRANSITION IN PHORBOL ESTER-TREATED MOUSE EGGS
Autore:
MOSES RM; KLINE D;
Indirizzi:
UNIV TORONTO,DEPT ZOOL,25 HARBORD ST TORONTO ON M5S 1A1 CANADA KENT STATE UNIV,DEPT BIOL SCI KENT OH 44242
Titolo Testata:
Developmental biology
fascicolo: 1, volume: 171, anno: 1995,
pagine: 111 - 122
SICI:
0012-1606(1995)171:1<111:CMSM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; INTRACELLULAR FREE CALCIUM; CELL-CYCLE; CHROMOSOME CONDENSATION; CHROMATID SEPARATION; XENOPUS EGGS; II ARREST; OOCYTES; ACTIVATION; 6-DIMETHYLAMINOPURINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Moses e D. Kline, "CALCIUM-INDEPENDENT, MEIOTIC SPINDLE-DEPENDENT METAPHASE-TO-INTERPHASE TRANSITION IN PHORBOL ESTER-TREATED MOUSE EGGS", Developmental biology, 171(1), 1995, pp. 111-122

Abstract

Mouse eggs, arrested at metaphase II of meiosis, form pronuclei as a result of fertilization or exposure to parthenogenetic agents, such asthe phorbol es ter phorbol myristate acetate (PMA). Exposure of eggs to the microtubule inhibitor colcemid caused the disappearance of the meiotic spindle and prevented the PMA-induced release from metaphase. However, colcemid- and PMA-treated eggs which lacked spindles formed nuclei when treated in addition with the protein synthesis inhibitor cycloheximide or the protein kinase inhibitor 6-dimethlyaminopurine. To prevent an increase in intracellular calcium concentration ([Ca2+](i)), eggs were exposed to the cell permeant acetoxymethyl ester (AIM) form of the calcium chelator 1,2-bis(o-aminophenoxy)ethane-N,N,N'N'-tetraacetic acid (BAPTA). Nuclei formed in 45% of eggs treated with BAPTA AM and PMA and in 90% of eggs treated with BAPTA AM, PMA, and cycloheximide, suggesting that an increase in [Ca2+](i) is not necessary for the PMA-induced release from metaphase. The [Ca2+](i) did not change in eggs which formed nuclei in response to PMA, providing additional evidence that PMA activates eggs without elevating the [Ca2+](i). (C) 1995Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:08:45