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Titolo:
SUBTYPE-SPECIFIC REGULATION OF MUSCARINIC RECEPTOR EXPRESSION AND FUNCTION BY HETEROLOGOUS RECEPTOR ACTIVATION
Autore:
JACKSON DA; NATHANSON NM;
Indirizzi:
UNIV WASHINGTON,DEPT PHARMACOL,BOX 357750 SEATTLE WA 98195 UNIV WASHINGTON,DEPT PHARMACOL SEATTLE WA 98195
Titolo Testata:
The Journal of biological chemistry
fascicolo: 38, volume: 270, anno: 1995,
pagine: 22374 - 22377
SICI:
0021-9258(1995)270:38<22374:SROMRE>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; EMBRYONIC CHICK HEART; CYCLIC-AMP FORMATION; ACETYLCHOLINE-RECEPTOR; PHOSPHATIDYLINOSITOL TURNOVER; CELLS; AGONISTS; CLONING; GENE; ANTAGONISTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
D.A. Jackson e N.M. Nathanson, "SUBTYPE-SPECIFIC REGULATION OF MUSCARINIC RECEPTOR EXPRESSION AND FUNCTION BY HETEROLOGOUS RECEPTOR ACTIVATION", The Journal of biological chemistry, 270(38), 1995, pp. 22374-22377

Abstract

Incubation of cultured embryonic chicken heart cells with the beta-adrenergic agonist isoproterenol resulted in a dose-dependent increase in the number of mAChR on the surface of intact cells. The isoproterenol-mediated increase in mAChR number was time dependent and reached a maximum by 48 h. Chick heart cells treated with isoproterenol exhibiteda greater than 6-fold increase in the sensitivity for carbachol-mediated inhibition of adenylyl cyclase activity as compared to control. Stimulation of cultured heart cells for 24 h with isoproterenol resultedin a 25-35% increase in cm2 mRNA levels as compared to control cm2 mRNA levels. In contrast, the level of cm4 mRNA was not significantly affected by isoproterenol treatment. cm2 mRNA levels were maximally elevated by 15 h following isoproterenol stimulation and remained elevatedfor up to 72 h. Incubation of cells with isoproterenol in the presence of Rp-cAMP, an inhibitor of cAMP-dependent protein kinase, blocked the increase in the level of cm2 mRNA. Thus, prolonged activation of beta-adrenergic receptors results in an increase in mAChR number and muscarinic responsiveness in chick heart cells due to a cAMP-dependent protein kinase mediated increase in cm2 mRNA levels.

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Documento generato il 30/11/20 alle ore 20:11:07