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Titolo:
COMPARISON OF CELL-PROLIFERATION FOLLOWING PARTIAL-HEPATECTOMY IN RATS FED NIH-31 OR SEMIPURIFIED AIN-76A DIETS - EFFECTS OF NUCLEIC-ACID SUPPLEMENTATION
Autore:
JACKSON CD; WEIS C; MILLER BJ; CROSS DR; WARBRITTON AR; JAMES SJ;
Indirizzi:
NATL CTR TOXICOL RES,DIV NUTR TOXICOL,3900 NCTR DR JEFFERSON AR 72079 NATL CTR TOXICOL RES,PATHOL ASSOCIATES INC JEFFERSON AR 72079
Titolo Testata:
Nutrition research
fascicolo: 10, volume: 15, anno: 1995,
pagine: 1487 - 1495
SICI:
0271-5317(1995)15:10<1487:COCFPI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICE; CARCINOGENESIS; NUCLEOTIDES; LIVER;
Keywords:
DIETARY NUCLEOTIDES; PARTIAL HEPATECTOMY; CELL PROLIFERATION; AIN-76A DIET;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
C.D. Jackson et al., "COMPARISON OF CELL-PROLIFERATION FOLLOWING PARTIAL-HEPATECTOMY IN RATS FED NIH-31 OR SEMIPURIFIED AIN-76A DIETS - EFFECTS OF NUCLEIC-ACID SUPPLEMENTATION", Nutrition research, 15(10), 1995, pp. 1487-1495

Abstract

Male Fischer 344 rats, were administered NIH-31, AIN-76A, or AIN-76A plus 0.25% yeast RNA diets for 3 weeks, partially hepatectomized (PH),and groups of 6 rats from each diet group were sacrificed at 0, 2, 7 and 14 days following PH. Liver weight, total DNA, total lipids, levels of thiobarbaturic acid reactive substances (TEARS), and levels of intracellular deoxyribonucleotides were determined. In addition, the number of hepatocytes in mitosis and/or positive for nuclear proliferating cell nuclear antigen (PCNA) were determined. Liver regeneration, measured as liver weight or total liver DNA, was equal in the three diet groups and was essentially complete by the end of 14 days. However, there was a 53% reduction in the mitotic index and a two-fold increase in the ratio of proliferating cells (G1 + S + G2 cells) to mitoses at 48 hours in animals of the AIN-76A diet group compared to those fed NIH-31 diet, suggesting that the cells were arrested or delayed in S-phase. At the time of PH, liver deoxyuridine monophosphate (dUMP) and deoxythymidine triphosphate (dTTP) levels in the AIN-76A group were decreased 29% and 57%, respectively, compared to those of the NIH-31 group. Addition of yeast RNA to the AIN-76A diet partially prevented the decrease in mitosis and maintained deoxyribonucleotide triphosphates (dNTP) levels to those of the NIH-31 group. These results indicate that thelack of dietary nucleotides in semipurified diets can alter dNTP levels and have adverse effects on cell proliferation. Consideration should be given to supplementing purified diets with a dietary source of nucleotides.

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Documento generato il 07/07/20 alle ore 12:47:38