Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INDUCTION OF PLASMODIUM-FALCIPARUM SPOROZOITE-NEUTRALIZING ANTIBODIESUPON VACCINATION WITH RECOMBINANT PFS16 VACCINIA VIRUS AND OR RECOMBINANT PFS16 PROTEIN PRODUCED IN YEAST/
Autore:
MOELANS IIMD; COHEN J; MARCHAND M; MOLITOR C; DEWILDE P; VANPELT JF; HOLLINGDALE MR; ROEFFEN WFG; ELING WMC; ATKINSON CT; AIKAWA M; SCHOENMAKERS JGG; KONINGS RNH;
Indirizzi:
JANSSEN PHARMACEUT,DEPT PARASITOL,TURNHOUTSEWEG 30 B-2340 BEERSE BELGIUM UNIV NIJMEGEN,FAC SCI,DEPT MOLEC BIOL NIJMEGEN NETHERLANDS SMITHKLINE BIOL,DEPT MOLEC & CELLULAR BIOL RIXENSART BELGIUM CATHOLIC UNIV LEUVEN,DEPT MED,DIV LIVER & PANCREAT DIS LOUVAIN BELGIUM US FDA ROCKVILLE MD 20857 UNIV NIJMEGEN,FAC MED,INST MED PARASITOL NIJMEGEN NETHERLANDS CASE WESTERN RESERVE UNIV,SCH MED,INST PATHOL CLEVELAND OH 44106
Titolo Testata:
Molecular and biochemical parasitology
fascicolo: 1-2, volume: 72, anno: 1995,
pagine: 179 - 192
SICI:
0166-6851(1995)72:1-2<179:IOPSA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CIRCUMSPOROZOITE PROTEIN; SACCHAROMYCES-CEREVISIAE; INFECTED HEPATOCYTES; PRIMARY CULTURES; MALARIA VACCINE; SURFACE-ANTIGEN; HEPATOMA-CELLS; MEMBRANE; RESPONSES; IMMUNITY;
Keywords:
PLASMODIUM FALCIPARUM; PFS16; RECOMBINANT VACCINIA VIRUS; HETEROLOGOUS PRODUCTION; YEAST; SPOROZOITE-NEUTRALIZING VACCINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
I.I.M.D. Moelans et al., "INDUCTION OF PLASMODIUM-FALCIPARUM SPOROZOITE-NEUTRALIZING ANTIBODIESUPON VACCINATION WITH RECOMBINANT PFS16 VACCINIA VIRUS AND OR RECOMBINANT PFS16 PROTEIN PRODUCED IN YEAST/", Molecular and biochemical parasitology, 72(1-2), 1995, pp. 179-192

Abstract

Pfs16 is a sexual stage/sporozoite-specific antigen of Plasmodium falciparum and is a potential candidate for a sporozoite-neutralizing vaccine. To obtain more information on the function of Pfs16 and to investigate its role during transmission and hepatocyte invasion, immunization experiments were performed with both a Pfs16-specific recombinant vaccinia virus and virus-like particles produced in yeast composed of the hepatitis B surface antigen (HBsAg) and antigen Pfs16 fused to HBsAg. Upon transformation of yeast cells, harbouring a genomic copy of the HBsAg gene, with a plasmid carrying the fusion gene Pfs16-HBsAg (Pfs16-S) virus-like hybrid particles composed of HBsAg and Pfs16-S were formed of a size similar to those present in human sera after infection with the hepatitis B virus. Cells infected with recombinant Pfs16 vaccinia virus synthesized a polypeptide of approx. 16 kDa that reacted with a Pfs16-specific polyclonal antibody. Animals vaccinated with theyeast hybrid particles and/or recombinant vaccinia virus both produced Pfs16-specific antibodies. These antibodies showed no transmission-blocking activity, but they efficiently diminished or abolished in vitro invasion of sporozoites into human hepatoma cells (HepG2-A16) and primary human hepatocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 02:16:12