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Titolo:
CONTROL OF THE IL-2 RESPONSIVENESS OF B-LYMPHOCYTES BY IL-2 AND IL-4
Autore:
MOREAU JL; CHASTAGNER P; TANAKA T; MIYASAKA M; KONDO M; SUGAMURA K; THEZE J;
Indirizzi:
INST PASTEUR,CELLULAR IMMUNOGENET UNIT,25 RUE DR ROUX F-75015 PARIS FRANCE INST PASTEUR,CELLULAR IMMUNOGENET UNIT F-75015 PARIS FRANCE OSAKA UNIV,SCH MED,BIOMED RES CTR,DEPT BIOREGULAT SUITA OSAKA 565 JAPAN TOHOKU UNIV,SCH MED,DEPT MICROBIOL SENDAI MIYAGI 980 JAPAN
Titolo Testata:
The Journal of immunology
fascicolo: 7, volume: 155, anno: 1995,
pagine: 3401 - 3408
SICI:
0022-1767(1995)155:7<3401:COTIRO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR BETA-CHAIN; INTERLEUKIN-2 RECEPTOR; MONOCLONAL-ANTIBODIES; GAMMA-CHAIN; T-CELLS; LYMPHOMA LINE; ALPHA-CHAIN; ANTI-MU; EXPRESSION; AFFINITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Moreau et al., "CONTROL OF THE IL-2 RESPONSIVENESS OF B-LYMPHOCYTES BY IL-2 AND IL-4", The Journal of immunology, 155(7), 1995, pp. 3401-3408

Abstract

A two-step culture system was used to analyze the parameters involvedin the acquisition of IL-2 responsiveness by murine B cells. In the first culture, unstimulated, or resting, B cells prepared from spleen of naive animals were challenged during 48 h with IL-2, IL-4, anti-mu, anti-mu + IL-2, anti-mu + IL-4, or anti-mu + IL-2 + IL-4. In a second culture, IL-2 responsiveness was followed by measuring either the cellproliferation or the Ig production. It was found that only B cells stimulated by anti-mu + IL-2 were able to respond. The expression of three chains of the IL-2 receptor (IL-2R alpha, IL-2R beta, and IL-2R gamma) was studied by FACS. IL-2R beta and IL-2R gamma were found to be expressed constitutively on resting B cells. IL-2R alpha was induced byanti-mu and anti-mu + IL-2 treatment. Although B cells treated by anti-mu alone are not able to respond to IL-2, they do express an IL-2 binding capacity comparable with B cells treated by anti-mu + IL-2. Thisparadoxical result suggests that IL-2 has a direct influence on the acquisition of the IL-2 responsiveness. IL-4 exerts a negative effect on the IL-2 response. At the molecular level, IL-4 was found to reduce selectively the IL-2R beta expression at the B cell surface. This effect was confirmed by Northern blot analysis. Maximum expression of the IL-2R beta mRNA is obtained after anti-mu + IL-2 treatment. In the presence of IL-4, expression of the IL-2R beta mRNA is greatly reduced.

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Documento generato il 28/01/20 alle ore 14:43:25