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Titolo:
CALCIUM-DEPENDENT TRANSMITTER SECRETION FROM FIBROBLASTS - MODULATIONBY SYNAPTOTAGMIN-I
Autore:
MORIMOTO T; POPOV S; BUCKLEY KM; POO MM;
Indirizzi:
COLUMBIA UNIV,DEPT BIOL SCI NEW YORK NY 10027 HARVARD UNIV,SCH MED,DEPT NEUROBIOL BOSTON MA 02115
Titolo Testata:
Neuron
fascicolo: 3, volume: 15, anno: 1995,
pagine: 689 - 696
SICI:
0896-6273(1995)15:3<689:CTSFF->2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROTRANSMITTER RELEASE; EXOCYTOSIS; ENDOCYTOSIS; MEMBRANE; NEURONS; DOCKING; CULTURE; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
T. Morimoto et al., "CALCIUM-DEPENDENT TRANSMITTER SECRETION FROM FIBROBLASTS - MODULATIONBY SYNAPTOTAGMIN-I", Neuron, 15(3), 1995, pp. 689-696

Abstract

Following endocytic uptake of acetylcholine (ACh), CHO fibroblasts exhibit Ca2+-dependent spontaneous quantal ACh release and depolarization-evoked ACh release, as detected by a whole-cell voltage-clamped myocyte in contact with the fibroblast. CHO fibroblasts transfected with synaptotagmin I, an integral membrane protein of synaptic vesicles, showed a reduced spontaneous quantal ACh release and an enhanced Ca2+-evoked ACh release, as compared with control cells. Biochemical and ultrastructural studies of endocytic activity using horseradish peroxidase as a marker further confirmed the inhibitory action of synaptotagmin Ion spontaneous vesicular exocytosis and on elevated exocytosis induced by Ca2+. Through inhibition of exocytosis at the resting intracellular concentration of Ca2+ and removal of the inhibition upon depolarization-induced Ca2+ entry, synaptotagmin I could enhance the efficiency of excitation-secretion coupling.

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Documento generato il 04/04/20 alle ore 22:28:26