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Titolo:
REACTIVE NITROGEN INTERMEDIATES IMPLICATED IN THE INHIBITION OF ENCEPHALITOZOON-CUNICULI (PHYLUM MICROSPORA) REPLICATION IN MURINE PERITONEAL-MACROPHAGES
Autore:
DIDIER ES;
Indirizzi:
TULANE REG PRIMATE RES CTR,DEPT MICROBIOL,18703 3 RIVERS RD COVINGTONLA 70433
Titolo Testata:
Parasite immunology
fascicolo: 8, volume: 17, anno: 1995,
pagine: 405 - 412
SICI:
0141-9838(1995)17:8<405:RNIIIT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE; NITRATE;
Keywords:
MICROSPORIDIA; E-CUNICULI; OPPORTUNISTIC PATHOGEN; MACROPHAGES NITROGEN INTER- MEDIATES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
E.S. Didier, "REACTIVE NITROGEN INTERMEDIATES IMPLICATED IN THE INHIBITION OF ENCEPHALITOZOON-CUNICULI (PHYLUM MICROSPORA) REPLICATION IN MURINE PERITONEAL-MACROPHAGES", Parasite immunology, 17(8), 1995, pp. 405-412

Abstract

Encephalitozoon cuniculi (phylum microspora) is a protozoan parasite that can replicate within parasitophorous vacuoles in macrophages. Thioglycollate-elicited BALB/c peritoneal macrophages treated with murinerecombinant interferon-gamma (rIFN-gamma; 100 u/ml) in combination with lipopolysaccharide (LPS; 10 ng/ml) for 24 h killed E. cuniculi as determined by significant reductions in the number of parasites and percent of infected macrophages 48 h later compared with cultures treatedwith medium only. Treatment of the elicited macrophages with murine rIFN-gamma (10 u/ml or 100 u/ml) only, resulted in microbistatic activity. Significantly higher levels of nitrite (NO2) were detected in supernatants from macrophage cultures treated with rIFN-gamma (10 u/ml or 100 u/ml) which induced microbistatic macrophage activity as well as from macrophage cultures treated with LPS + rIFN- when compared with levels of nitrite detected in supernatants of infected ariginine analogue, N-3 monomethyl-L-arginine (NMMA) at concentrations of 50, 100 or 250 uM significantly inhibited nitrite synthesis and prevented microsporidia killing. Addition of exogenous L-arginine at concentrations of 5 mm or 10 mm reversed the NMMA-induced inhibition of parasite killing. These results indicate that reactive nitrogen intermediates contributeto the killing of E. cuniculi by LPS + rIFN-gamma-activated murine peritoneal macrophages in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 18:04:32