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Titolo:
A COMBINED INDIRECT ELISA AND IMMUNOBLOTTING FOR THE DETECTION OF INTRATHECAL HERPES-SIMPLEX VIRUS IGG ANTIBODY-SYNTHESIS IN PATIENTS WITH HERPES-SIMPLEX VIRUS ENCEPHALITIS
Autore:
MARKOULATOS P; LABROPOULOU V; KORDOSSI A; KRIKELIS V; SPYROU N; MONCANY MLJ;
Indirizzi:
UNIV LA ROCHELLE,MOLEC & CELLULAR BIOL LAB,AVE MARILLAC F-17042 LA ROCHELLE FRANCE UNIV LA ROCHELLE,MOLEC & CELLULAR BIOL LAB F-17042 LA ROCHELLE FRANCE SEYP,TEI,DEPT MED LABS LARISA GREECE HELLEN INST PASTEUR ATHENS GREECE
Titolo Testata:
Journal of clinical laboratory analysis
fascicolo: 5, volume: 9, anno: 1995,
pagine: 325 - 333
SICI:
0887-8013(1995)9:5<325:ACIEAI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBROSPINAL-FLUID; EARLY DIAGNOSIS; ACYCLOVIR; VIDARABINE; PROTEINS; SERUM; CELLS; DNA;
Keywords:
HERPES SIMPLEX VIRUS (HSV); HSV ENCEPHALITIS; SERUM; CEREBROSPINAL FLUID (CSF); ELISA; IMMUNOBLOTTING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
P. Markoulatos et al., "A COMBINED INDIRECT ELISA AND IMMUNOBLOTTING FOR THE DETECTION OF INTRATHECAL HERPES-SIMPLEX VIRUS IGG ANTIBODY-SYNTHESIS IN PATIENTS WITH HERPES-SIMPLEX VIRUS ENCEPHALITIS", Journal of clinical laboratory analysis, 9(5), 1995, pp. 325-333

Abstract

A combined indirect ELISA and immuno-blotting assay was used for the detection of intrathecal synthesis of IgG antibodies to herpes simplexvirus (HSV) in patients with HSV encephalitis (HSVE). By using these two assays as well as three markers for blood-brain barrier, leakage can be easily excluded. A total of 21 sera and 24 cerebrospinal fluid (CSF) samples from 11 patients with HSVE were examined. For seven patients more than one pair of serum and CSF were available. For one patient IgG antibodies began to be detectable in CSF after the sixth day from the onset of the disease. In the other 10 patients the intrathecal synthesis of HSV IgG antibodies was detected later than the sixth day and reached high optical density (OD) values after the 10th day from the onset of disease, at the earliest. In contrast, intrathecal HSV antibody synthesis was not found in specimens taken from 20 patients with acute meningitis who composed our negative control group. The use of acombined indirect ELISA and of an immunoblotting assay on a single dilution of serum and CSF for HSV IgG synthesis in the central nervous system (CNS) allowed the diagnosis of HSVE after the first week of disease. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:31:46