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Titolo:
PERSISTENT NA-SIZED NEOSTRIATAL NEURONS - CHARACTERIZATION USING INFRARED VIDEOMICROSCOPY AND WHOLE-CELL PATCH-CLAMP RECORDINGS( CONDUCTANCE IN MEDIUM)
Autore:
CEPEDA C; CHANDLER SH; SHUMATE LW; LEVINE MS;
Indirizzi:
UNIV CALIF LOS ANGELES,MENTAL RETARDAT RES CTR,760 WESTWOOD PLAZA LOSANGELES CA 90024 UNIV CALIF LOS ANGELES,MENTAL RETARDAT RES CTR LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,DEPT PHYSIOL SCI LOS ANGELES CA 90024
Titolo Testata:
Journal of neurophysiology
fascicolo: 3, volume: 74, anno: 1995,
pagine: 1343 - 1348
SICI:
0022-3077(1995)74:3<1343:PNNN-C>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVITRO SLICE PREPARATION; CENTRAL NERVOUS-SYSTEM; SODIUM CURRENT; ELECTROPHYSIOLOGICAL PROPERTIES; SENSORIMOTOR CORTEX; HIPPOCAMPAL-NEURONS; NEOCORTICAL NEURONS; MEMBRANE-PROPERTIES; PYRAMIDAL NEURONS; CAUDATE NEURONS;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Physical, Chemical & Earth Sciences
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
C. Cepeda et al., "PERSISTENT NA-SIZED NEOSTRIATAL NEURONS - CHARACTERIZATION USING INFRARED VIDEOMICROSCOPY AND WHOLE-CELL PATCH-CLAMP RECORDINGS( CONDUCTANCE IN MEDIUM)", Journal of neurophysiology, 74(3), 1995, pp. 1343-1348

Abstract

1. In the present study we investigate the expression of a persistentNa+ conductance (I-NaP) in identified medium-sized neostriatal neurons. Nomarski optics and infrared videomicroscopy were used for cell visualization and identification in thick slices (350 mu m). Current- andvoltage-clamp recordings were obtained utilizing whole cell patch-clamp methodology. 2. Application of depolarizing ramp voltage commands from a holding potential of -70 mV induced a slow, noninactivating inward current that occurred before and independent of the rapidly inactivating sodium current that subserves action potential generation. I-NaPbegan to activate at potentials less negative than -70 mV and peaked at -34 +/- 1 (SE) mV. Its average peak amplitude was -100 +/- 17 pA. I-NaP was abolished by tetrodotoxin (TTX, 0.5-1 mu M) or an Na+-free solution. In contrast, it was not affected by Ca2+ channel blockers. Depolarizing ramp commands also induced tetraethylammonium-sensitive outward currents. 3. Dopamine (DA) (20-100 mu M) produced a significant reduction of I-NaP. 4. These results demonstrate the existence of a TTX-sensitive persistent Na+ conductance in medium-sized neostriatal neurons. This conductance is modulated by DA and could play a role in the generation of rhythmic oscillations and in supporting repetitive firing.

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Documento generato il 05/12/20 alle ore 01:27:09