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Titolo:
GENOTYPIC IDENTIFICATION OF 2 GROUPS WITHIN THE SPECIES BACTEROIDES-FRAGILIS BY RIBOTYPING AND BY ANALYSIS OF PCR-GENERATED FRAGMENT PATTERNS AND INSERTION-SEQUENCE CONTENT
Autore:
PODGLAJEN I; BREUIL J; CASIN I; COLLATZ E;
Indirizzi:
UNIV PARIS 06,RECH MOLEC ANTIBIOT LAB,15 RUE ECOLE MED F-75270 PARIS 06 FRANCE UNIV PARIS 06,RECH MOLEC ANTIBIOT LAB F-75270 PARIS 06 FRANCE UNIV PARIS 07,HOP ST LOUIS PARIS FRANCE CTR HOSP INTERCOMMUNAL VILLENEUVE ST GEO FRANCE
Titolo Testata:
Journal of bacteriology
fascicolo: 18, volume: 177, anno: 1995,
pagine: 5270 - 5275
SICI:
0021-9193(1995)177:18<5270:GIO2GW>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
METALLO-BETA-LACTAMASE; UNITED-STATES; RIBOSOMAL-RNA; IMIPENEM; GENE; SUSCEPTIBILITY; DNA; RESISTANCE; STRAINS; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
I. Podglajen et al., "GENOTYPIC IDENTIFICATION OF 2 GROUPS WITHIN THE SPECIES BACTEROIDES-FRAGILIS BY RIBOTYPING AND BY ANALYSIS OF PCR-GENERATED FRAGMENT PATTERNS AND INSERTION-SEQUENCE CONTENT", Journal of bacteriology, 177(18), 1995, pp. 5270-5275

Abstract

Molecular typing allowed the separation of the species Bacteroides fragilis into two genotypically distinct groups. A unique set of 50 strains of B. fragilis carrying the chromosomal metallo-beta-lactamase gene cfiA was subjected to a comparative analysis with respect to sets ofup to 250 randomly collected strains devoid of this gene. The two groups were found to be distinct on the basis of the following results: (i) ribotyping, after DNA digestion with AvaI, revealed a practically homogeneous DNA fragment pattern for the cfiA-positive strains and distinct multiple patterns for the cfiA-negative strains; (ii) PCR, arbitrarily primed with an experimentally selected decamer, generated fragment patterns typical for the strains of each group; (iii) the three insertion sequences described to date in the species B. fragilis, i.e., IS4351, IS942, and IS1186, were all but confined to the cfiA-positive group, in which they were capable of providing promoter sequences for the transcription of cfiA; and (iv) the cepA gene, encoding the so-called endogenous cephalosporinase of B. fragilis was found exclusively inthe cfiA-negative group, in which it was present in ca. 70% of the strains. The cfiA-, cepA-negative fraction was not characterized Further. In a natural population of 500 randomly selected strains of B. fragilis, the cfiA positive and cfiA-negative groups represented ca. 3 and 97% of the strains, respectively. Analysis of 82 metabolic traits revealed no difference between the two groups.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:43:45