Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHARACTERIZATION OF DELETION MUTATIONS IN THE CAPSID REGION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 THAT AFFECT PARTICLE FORMATION AND GAG-POL PRECURSOR INCORPORATION
Autore:
SRINIVASAKUMAR N; HAMMARSKJOLD ML; REKOSH D;
Indirizzi:
UNIV VIRGINIA,MYLES H THALER CTR AIDS & HUMAN RETROVIRUS RES CHARLOTTESVILLE VA 22908 UNIV VIRGINIA,MYLES H THALER CTR AIDS & HUMAN RETROVIRUS RES CHARLOTTESVILLE VA 22908 UNIV VIRGINIA,DEPT MICROBIOL CHARLOTTESVILLE VA 22908
Titolo Testata:
Journal of virology
fascicolo: 10, volume: 69, anno: 1995,
pagine: 6106 - 6114
SICI:
0022-538X(1995)69:10<6106:CODMIT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
LATE REPLACEMENT VECTOR; BACULOVIRUS-INFECTED CELLS; OVERLAP EXTENSION; EXPRESSION; PR160GAG-POL; PR55GAG; MUTANTS; OVEREXPRESSION; REPLICATION; INFECTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
N. Srinivasakumar et al., "CHARACTERIZATION OF DELETION MUTATIONS IN THE CAPSID REGION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 THAT AFFECT PARTICLE FORMATION AND GAG-POL PRECURSOR INCORPORATION", Journal of virology, 69(10), 1995, pp. 6106-6114

Abstract

The core of human immunodeficiency virus type 1 is derived from two precursor polyproteins, Pr55(gag) and Pr160(gag-pol). The Gag precursorcan assemble into immature virus-like particles when expressed by itself, while the Gag-Pol precursor lacks particle-forming ability, We have shown previously that the Gag precursor is able to ''rescue'' the Gag-Pol precursor into virus-like particles when the two polyproteins are expressed in the same cell by using separate simian virus 40-based plasmid expression vectors. To understand this interaction in greater detail, we have made deletion mutations in the capsid-coding regions of Gag- and Gag-Pol-expressing plasmids and assayed for the abilities of these precursors to assemble into virus-like particles. When we tested the abilities of Gag-Pol precursors to be incorporated into particles of Gag by coexpressing the precursors, we found that mutant Gag-Polprecursors lacking a conserved region in retroviral capsid proteins, the major homology region (MHR), were excluded from wild-type Gag particles, Mutant precursors lacking MHR were also less efficient in processing the Gag precursor in trans. These results suggest that the MHR is critical for interactions between Gag and Gag-Pol molecules. In contrast to these results, expression of mutated Gag precursors alone showed that deletions in the capsid region, including those which removed the MHR, reduced the efficiency of particle formation by only 40 to 50%, The mutant particles, however, were clearly lighter than the wild type in sucrose density gradients. These results indicate that the requirements for Gag particle formation differ from the ones essential forefficient incorporation of the Gag-Pol precursor into these particles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:21:59