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Titolo:
LIGAND-INDUCED ENDOCYTOSIS OF EPIDERMAL GROWTH-FACTOR RECEPTORS THAT ARE DEFECTIVE IN BINDING ADAPTER PROTEINS
Autore:
NESTEROV A; WILEY HS; GILL GN;
Indirizzi:
UNIV CALIF SAN DIEGO,SCH MED,DEPT MED LA JOLLA CA 92093 UNIV CALIF SAN DIEGO,SCH MED,DEPT MED LA JOLLA CA 92093 UNIV UTAH,SCH MED,DEPT PATHOL SALT LAKE CITY UT 84123
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 19, volume: 92, anno: 1995,
pagine: 8719 - 8723
SICI:
0027-8424(1995)92:19<8719:LEOEGR>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYSOSOMAL ACID-PHOSPHATASE; CARCINOMA-CELLS A-431; INDUCED INTERNALIZATION; MEDIATED ENDOCYTOSIS; INVITRO BINDING; COATED VESICLES; KINASE-ACTIVITY; A431 CELLS; CLATHRIN; TRANSFERRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
A. Nesterov et al., "LIGAND-INDUCED ENDOCYTOSIS OF EPIDERMAL GROWTH-FACTOR RECEPTORS THAT ARE DEFECTIVE IN BINDING ADAPTER PROTEINS", Proceedings of the National Academy of Sciences of the United Statesof America, 92(19), 1995, pp. 8719-8723

Abstract

Ligand-activated epidermal growth factor receptors (EGFRs) associate with coated pit adaptor proteins (AP2) in vivo, implying a mechanism for receptor retention in coated pits during internalization. Using an in vitro binding assay, we localized the adaptor binding determinant to residues 970-991 of EGFRs and confirmed specificity by competition with a synthetic peptide corresponding to this sequence. A mutant EGFR lacking this AP2 binding determinant did not associate with AP2 in vivo but demonstrated internalization and down-regulation kinetics indistinguishable from its wildtype counterpart. Immunocytochemistry confirmed ligand-induced internalization of the mutant EGFR. These data suggest that endocytic determinants are distinct from AP2 binding determinants and that processes other than association with AP2 regulate endocytosis of EGFRs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 00:51:05