Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
BOUND FIBRINOGEN DISTRIBUTION ON STIMULATED PLATELETS - EXAMINATION BY CONFOCAL SCANNING LASER MICROSCOPY
Autore:
PEERSCHKE EIB;
Indirizzi:
SUNY STONY BROOK,UNIV HOSP,L-3 STONY BROOK NY 11794 SUNY STONY BROOK,DEPT PATHOL STONY BROOK NY 11794
Titolo Testata:
The American journal of pathology
fascicolo: 3, volume: 147, anno: 1995,
pagine: 678 - 687
SICI:
0002-9440(1995)147:3<678:BFDOSP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE-ACTIVATED PLATELETS; RECEPTOR LIGAND COMPLEXES; MEMBRANE GLYCOPROTEIN-IIB; MONOCLONAL-ANTIBODIES; IIIA COMPLEX; BINDING; REDISTRIBUTION; AGGREGATION; INHIBITION; ADHESION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
E.I.B. Peerschke, "BOUND FIBRINOGEN DISTRIBUTION ON STIMULATED PLATELETS - EXAMINATION BY CONFOCAL SCANNING LASER MICROSCOPY", The American journal of pathology, 147(3), 1995, pp. 678-687

Abstract

Previous studies have suggested that qualitative changes in platelet bound fibrinogen modulate platelet aggregation. The present study usedconfocal scanning laser microscopy to further evaluate post-ligand binding events over a 60-minute time course. When fluorescein isothiocyanate (FITC)-streptavidin was added to ADP-stimulate platelets 1 minuteafter biotinylated fibrinogen binding at 22 degrees C, bound fibrinogen was found in variously sized patches on the cell surface. When streptavidin was added 60 minutes later, bound fibrinogen had been clearedfrom the platelet surface and was observed in clusters penetrating into platelets to various extents. ADP-activated platelets did not stainwith a monoclonal antibody against CD62 suggesting that platelets were not permeabilized during the experiment and had not released alpha-granules. Additional studies using either biotinylated fibrinogen that had been prelabeled with FITC-streptavidin or FITC-labeled fibrinogen revealed similar patterns of platelet-associated fibrinogen clearance and redistribution. Pretreatment of platelets with cytochalasin D prevented this redistribution. Dual labeling experiments using biotinylated fibrinogen and FITC-streptavidin as well as a monoclonal anti-GPIIIaantibody labeled with rhodamine-conjugated anti-mouse IgG demonstrated the co-localization of fibrinogen and GPIIIa. Similar observations were made with fibrinogen bound to thrombin-stimulated platelets. In contrast, fibronectin bound to thrombin-activated platelets retained a predominantly surface membrane distribution under identical experimental conditions. Since surface-cleared fibrinogen was accessible to exogenous FITC-streptividin under conditions that did not lead to platelet permeabilization, the data suggest fibrinogen deposition in compartments that are accessible to the extracellular milieu. This is consistentwith the ability of exogenous plasmin to completely remove cleared fibrinogen pools without detectable fibrinogen reexpression on the platelet surface or alpha-granule secretion. The data provide morphologicalevidence for the selectivity, GPIIb-IIIa mediated, actin-dependent clearance of bound fibrinogen from the activated platelet surface, suggesting a mechanism for preventing and limiting thrombus development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 02:13:46