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Titolo:
A PLASMID-BASED SELF-AMPLIFYING SINDBIS-VIRUS VECTOR
Autore:
HERWEIJER H; LATENDRESSE JS; WILLIAMS P; ZHANG GF; DANKO I; SCHLESINGER S; WOLFF JA;
Indirizzi:
UNIV WISCONSIN,WAISMAN CTR 355,DEPT PEDIAT & MED GENET,1500 HIGHLAND AVE MADISON WI 53705 UNIV WISCONSIN,WAISMAN CTR 355,DEPT PEDIAT & MED GENET MADISON WI 53705 WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL ST LOUIS MO 63110
Titolo Testata:
Human gene therapy
fascicolo: 9, volume: 6, anno: 1995,
pagine: 1161 - 1167
SICI:
1043-0342(1995)6:9<1161:APSSV>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECT GENE-TRANSFER; EXPRESSION VECTORS; RNA-POLYMERASE; SKELETAL-MUSCLE; MOUSE MUSCLE; CELLS; EFFICIENCY; MYOTUBES; INVIVO; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
H. Herweijer et al., "A PLASMID-BASED SELF-AMPLIFYING SINDBIS-VIRUS VECTOR", Human gene therapy, 6(9), 1995, pp. 1161-1167

Abstract

Sindbis virus was used as a self-amplifying eukaryotic expression vector. A recombinant cDNA genome of this (+)-strand RNA virus was placedunder the transcriptional control of a Rous sarcoma virus LTR (RSV) promoter. Transfection of this plasmid construct into mammalian cell lines (3T3, HepG2, and 293 cells) resulted in expression of the luciferase reporter gene. High-expression levels were also measured after transfection into primary rat myoblasts. In differentiated myotubes, expression levels generated by the Sindbis virus vector were up to 200 times higher than those obtained with a conventional RSV expression vector. In vivo expression was detected after injection of plasmid DNA into mouse quadriceps. In vivo expression was transient and undetectable byday 16. This self-amplifying expression vector can be used for generating high-level expression of transgenes in vitro and in vivo. Its transient nature in vivo could allow for safe, short-term delivery of gene products in gene therapy protocols. It should facilitate the study of Sindbis and other RNA viruses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:20:34