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Titolo:
ROLE OF 5-HYDROXYTRYPTAMINE TYPE-3 RECEPTORS IN RAT INTESTINAL FLUID AND ELECTROLYTE SECRETION INDUCED BY CHOLERA AND ESCHERICHIA-COLI ENTEROTOXINS
Autore:
MOURAD FH; ODONNELL LJD; DIAS JA; OGUTU E; ANDRE EA; TURVILL JL; FARTHING MJG;
Indirizzi:
UNIV LONDON ST BARTHOLOMEWS HOSP & MED COLL,DIGEST DIS RES CTR,CHARTERHOUSE SQ LONDON EC1M 6BQ ENGLAND UNIV LONDON ST BARTHOLOMEWS HOSP & MED COLL,DIGEST DIS RES CTR LONDONEC1M 6BQ ENGLAND
Titolo Testata:
Gut
fascicolo: 3, volume: 37, anno: 1995,
pagine: 340 - 345
SICI:
0017-5749(1995)37:3<340:RO5TRI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-STABLE ENTEROTOXIN; ENTERIC NERVOUS-SYSTEM; ION-TRANSPORT; CYCLIC-GMP; TOXIN; RELEASE; INVIVO; MODEL; INVOLVEMENT; ANTAGONISTS;
Keywords:
5-HYDROXYTRYPTAMINE; CHOLERA TOXIN; ESCHERICHIA-COLI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
F.H. Mourad et al., "ROLE OF 5-HYDROXYTRYPTAMINE TYPE-3 RECEPTORS IN RAT INTESTINAL FLUID AND ELECTROLYTE SECRETION INDUCED BY CHOLERA AND ESCHERICHIA-COLI ENTEROTOXINS", Gut, 37(3), 1995, pp. 340-345

Abstract

Cholera toxin and Escherichia coli heat labile toxin (LT) induced intestinal secretion has in the past been attributed exclusively to an increase in intracellular cAMP whereas E coli heat stable toxin (ST) induced secretion is mediated through cGMP. Evidence is accumulating on the importance of 5-hydroxytryptamine (5-HT) in cholera toxin induced secretion, but its role in LT and ST is not well established. This study therefore investigated in vivo the effect of 5-HT3 receptor antagonist, granisetron, on intestinal fluid and electrolyte secretion inducedby cholera toxin, LT, and ST. Granisetron (30, 75, 150, or 300 mu g/kg) was given subcutaneously to adult male Wistar rats 90 minutes before instillation of 75 mu g cholera toxin or 50 mu g LT in isolated whole small intestine. In situ small intestinal perfusion was performed with an iso-osmotic plasma electrolyte solution (PES) to assess fluid movement. In a second group of animals, granisetron (300 mu g/kg) was given subcutaneously and two hours later small intestinal perfusion withPES containing 200 mu g/l ST was performed. Cholera toxin induced netfluid secretion (median -50.1 mu l/min/g (interquartile range -59.5 to -29.8)) was found to be dose dependently decreased or abolished by granisetron (plateau effect at 75 mu g/kg: 18 (-7.8 to 28), p<0.01). Granisetron in high dose (300 mu g/kg) however, failed to prevent LT or ST induced secretion (-52 (-121 to -71) upsilon -31 (-44 to -18), and (-39 (-49 to 17) upsilon (-22 (-39 to -3)), respectively). Sodium and chloride movement paralleled that of fluid. In conclusion, these data show that 5-HT and 5-HT3 receptors play an important part in cholera toxin induced secretion but are not involved in E coli heat stable or heat labile toxin induced secretion

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Documento generato il 02/12/20 alle ore 14:57:29