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Titolo:
THE EFFECTS OF A SIMPLIFIED METHOD FOR CRYOPRESERVATION AND THAWING PROCEDURES ON PERIPHERAL-BLOOD STEM-CELLS
Autore:
KATAYAMA Y; YANO T; BESSHO A; DEGUCHI S; SUNAMI K; MAHMUT N; SHINAGAWA K; OMOTO E; MAKINO S; MIYAMOTO T; MIZUNO S; FUKUDA T; ETO T; FUJISAKI T; OHNO Y; INABA S; NIHO Y; HARADA M;
Indirizzi:
OKAYAMA UNIV,SCH MED,DEPT INTERNAL MED 2,2-5-1 SHIKATA CHO OKAYAMA 700 JAPAN KYUSHU UNIV HOSP,BLOOD TRANSFUS SERV FUKUOKA 812 JAPAN KYUSHU UNIV,FAC MED,DEPT INTERNAL MED 1 FUKUOKA 812 JAPAN
Titolo Testata:
Bone marrow transplantation
fascicolo: 3, volume: 19, anno: 1997,
pagine: 283 - 287
SICI:
0268-3369(1997)19:3<283:TEOASM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; HUMAN-BONE-MARROW; DIMETHYLSULFOXIDE; TOXICITY;
Keywords:
SIMPLIFIED CRYOPRESERVATION OF PBSC; LONG-TERM CRYOPRESERVATION; DMSO EXPOSURE; SLOW THAWING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
Y. Katayama et al., "THE EFFECTS OF A SIMPLIFIED METHOD FOR CRYOPRESERVATION AND THAWING PROCEDURES ON PERIPHERAL-BLOOD STEM-CELLS", Bone marrow transplantation, 19(3), 1997, pp. 283-287

Abstract

A simplified method for cryopreservation at -80 degrees C of peripheral blood stem cells (PBSC) has been increasingly used for autologous PBSC transplantation in Japan, Although this method, using 6% hydroxyethyl starch (HES) and 5% dimethyl sulfoxide (DMSO) as a cryoprotectant without rate-controlled freezing, has several advantages over the conventional method using 10% DMSO with rate-controlled freezing, little is known about effects of long-term cryopreservation for Sears and thawing process on hematopoietic progenitors, We examined the recovery rates of BFU-E and CFU-GM in sample tubes cryopreserved by the simplifiedmethod under various conditions as follows: (1) long-term storage for1-5 years; (2) DMSO exposure for 1 h after rapid thawing; and (3) thawing at a lower temperature other than 37 degrees C, In our study, we found that the recovery rates of BFU-E and CFU-GM were not affected bythe length of cryopreservation period; they remained at more than 70%on average for 16-61 months. In our hands, a l-h exposure to DMSO after rapid thawing was not toxic for hematopoietic progenitors, Furthermore, there was no significant difference in the recovery rates of BFU-E and CFU-GM between thawing at 37 degrees C and 20 degrees C, These observations indicate that PBSC cryopreserved for at least 5 years by the simplified method can be used clinically without losing hematopoietic activity, and suggest that hematopoietic activity of the thawed PBSC may be unaffected when PBSC are infused slowly within 60 min or evenwhen PBSC are thawed gradually at room temperature.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 17:58:46