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Titolo:
EVIDENCE FOR AN ENDOGENOUS OUABAIN-LIKE IMMUNOREACTIVE FACTOR IN HUMAN NEWBORN PLASMA COELUTED WITH OUABAIN ON HPLC
Autore:
DIBARTOLO V; BALZAN S; PIERACCINI L; GHIONE S; PEGORARO S; BIVER P; REVOLTELLA R; MONTALI U;
Indirizzi:
UNIV PISA,INST BIOL CHEM,VIA SAVI 55 I-56126 PISA ITALY UNIV PISA,INST BIOL CHEM I-56126 PISA ITALY UNIV PISA,DEPT NEONATOL I-56126 PISA ITALY UNIV PISA,CNR,INST MUTAGENESIS & DIFFERENTIAT I-56126 PISA ITALY UNIV PISA,CNR,INST CLIN PHYSIOL I-56126 PISA ITALY
Titolo Testata:
Life sciences
fascicolo: 15, volume: 57, anno: 1995,
pagine: 1417 - 1425
SICI:
0024-3205(1995)57:15<1417:EFAEOI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIGITALIS-LIKE-FACTOR; DIGOXIN-LIKE IMMUNOREACTIVITY; CORD BLOOD; CHROMATOGRAPHY; HYPERTENSION; PURIFICATION; INHIBITOR; EXTRACTS; SERUM;
Keywords:
ENDOGENOUS DIGITALIS-LIKE FACTOR; HUMAN NEWBORN PLASMA; OUABAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
V. Dibartolo et al., "EVIDENCE FOR AN ENDOGENOUS OUABAIN-LIKE IMMUNOREACTIVE FACTOR IN HUMAN NEWBORN PLASMA COELUTED WITH OUABAIN ON HPLC", Life sciences, 57(15), 1995, pp. 1417-1425

Abstract

The identification in human plasma of ouabain as an endogenous digitalis-like factor (EDLF) claimed by Hamlyn et al. has recently been contradicted by two studies which failed to demonstrate endogenous ouabain-like immunoreactivity in HPLC fractions in which exogenous ouabain was eluted. In this paper we report the results obtained on the cross-reactivity with antiouabain antibodies of an EDLF purified by us from human newborn cord plasma. We found that this EDLF coeluted with ouabainon HPLC and cross-reacted both with rabbit anti-ouabain antiserum andwith the purified antibodies, which excluded possible interferences due to antibodies directed against non-ouabain portions of the immunogen. Similar but not identical slopes of the ouabain and EDLF displacements curves were observed in all competition ELISA experiments. The inhibitory effect of EDLF on erythrocyte Rb-86 uptake was reversed by antiouabain antiserum and antibodies. The concentration of EDLF in newborn plasma, in the four different purifications studied ranged from 30 to 380 pM ouabain equivalents (o.e.) by ELISA and from 100 to 300 pM o.e. by Rb-86 uptake. Our data thus support the existence, in human newborn plasma, of a factor with both biological and immunological ouabain-like properties, although not necessarily identical to ouabain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:43:40