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Titolo:
PITUITARY PRL SECRETION INDUCED BY TETRAETHYLAMMONIUM IS INHIBITED BYDOPAMINE THROUGH D-2 RECEPTORS
Autore:
WANG XB; SATO N; GREER MA; FALARDEAU P;
Indirizzi:
OREGON HLTH SCI UNIV,DEPT PHYSIOL,ENDOCRINOL SECT PORTLAND OR 97201 OREGON HLTH SCI UNIV,DEPT PHYSIOL,ENDOCRINOL SECT PORTLAND OR 97201 OREGON HLTH SCI UNIV,DEPT MED PORTLAND OR 97201 CHUL,CTR RECH,DEPT MED GENET & MOLEC ST FOY PQ G1V 4G2 CANADA UNIV LAVAL,SCH PHARM ST FOY PQ G1V 4G2 CANADA
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 2, volume: 112, anno: 1995,
pagine: 153 - 157
SICI:
0303-7207(1995)112:2<153:PPSIBT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYROTROPIN-RELEASING-HORMONE; RAT LACTOTROPH CELLS; HIGH MEDIUM POTASSIUM; PROLACTIN SECRETION; GH4C1 CELLS; CALCIUM INFLUX; G-PROTEIN; CHANNELS; CURRENTS; ADENOSINE-3',5'-MONOPHOSPHATE;
Keywords:
PITUITARY; PROLACTIN SECRETION; DOPAMINE D-2 RECEPTORS; G-PROTEIN; CALCIUM CHANNELS; TETRAETHYLAMMONIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
X.B. Wang et al., "PITUITARY PRL SECRETION INDUCED BY TETRAETHYLAMMONIUM IS INHIBITED BYDOPAMINE THROUGH D-2 RECEPTORS", Molecular and cellular endocrinology, 112(2), 1995, pp. 153-157

Abstract

We have evaluated the inhibitory effect of dopamine on PRL secretion induced by blocking K+ channels. Tumor-derived GH(4)C(1) cells and collagenase-dispersed normal anterior pituitary (AP) cells from young adult male rats were perifused with Krebs-Ringer Hepes medium. In both cell types blocking K+ channels with tetraethylammonium (TEA) induced PRL secretion but did not stimulate cyclic AMP generation. Blocking Na+ channels with 1 mu M tetrodotoxin had no effect on basal or TEA-induced PRL secretion. Dopamine inhibited the TEA-induced rise in [Ca2+](i) in GH(4)C(1) cells expressing dopamine D-2 short receptors. In normal AP cells, 1-100 nM dopamine blocked PRL secretion induced by 20 mM TEAin a log-linear concentration-dependent fashion, with a plateau at > 100 nM dopamine (IC50 30 nM). The D-2 dopaminergic receptor agonist, quinpirole, at 100 nM completely blocked PRL secretion induced by 20 mMTEA. The D-2 dopaminergic receptor antagonist, sulpiride, at 10 mu M reversed the inhibitory effect of 10 mu M dopamine on PRL secretion induced by 20 mM TEA. Pretreatment of cells with 100 ng/ml pertussis toxin (PTX) for 24 h prevented 100 nM dopamine inhibition of PRL secretion induced by 20 mM TEA. The data indicate that in both normal lactotroph cells and in tumor-derived cells expressing D-2 receptors, PRL secretion stimulated by blocking K+ channels is inhibited by dopamine binding to D-2 receptors on the plasma membrane. This inhibition involves interaction with PTX-sensitive G(i) protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:12:07