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Titolo:
MUSCLE GENE E-BOX CONTROL ELEMENTS - EVIDENCE FOR QUANTITATIVELY DIFFERENT TRANSCRIPTIONAL ACTIVITIES AND THE BINDING OF DISTINCT REGULATORY FACTORS
Autore:
APONE S; HAUSCHKA SD;
Indirizzi:
UNIV WASHINGTON,DEPT BIOCHEM,SJ-70 SEATTLE WA 98195
Titolo Testata:
The Journal of biological chemistry
fascicolo: 36, volume: 270, anno: 1995,
pagine: 21420 - 21427
SICI:
0021-9258(1995)270:36<21420:MGECE->2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HELIX-LOOP-HELIX; CREATINE-KINASE GENE; MYOGENIC DETERMINATION FACTORS; DNA-BINDING; ENHANCER; PROTEIN; MYOD; ACTIVATION; SKELETAL; SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
S. Apone e S.D. Hauschka, "MUSCLE GENE E-BOX CONTROL ELEMENTS - EVIDENCE FOR QUANTITATIVELY DIFFERENT TRANSCRIPTIONAL ACTIVITIES AND THE BINDING OF DISTINCT REGULATORY FACTORS", The Journal of biological chemistry, 270(36), 1995, pp. 21420-21427

Abstract

The muscle creatine kinase gene enhancer contains two regulatory elements (MCK-R and MCK-L) with the consensus E-box sequence (CAnnTG). A myocyte specific protein complex, MEF1, binds the MCK-R site. MEF1 contains several basic H-L-H myogenic determination factors (MDFs), each dimerized with ubiquitous members of the bH-L-H family (e.g. E12/E47). We now demonstrate that the ubiquitous bH-L-H factor E2-2 is a major component of the endogenous MCK-R site specific complex. Previous studies described the MCK-L site as a similar but low affinity MDF/bH-L-H heterodimer binding site. However, we find that the MCK-L site exhibitsprefer ential binding of an unknown ubiquitous factor which contains neither E12/E47 nor E2-2, and that it exhibits differential transcriptional activity with muscle and non-muscle cells. The differential behavior of the MCK-L and MCK-R sites may be a general trait of E-box elements since one among several E-boxes in the MLC 1/3 enhancer also binds preferentially to the MCK-L factor. From our studies we now propose separate consensus sequences for MCK-R and MCK-L E-box types: AACAc/gc/gTGCa/t and GGa/cCANGTGGc/gNa/g. Our results suggest that while many muscle gene E-boxes are capa ble of binding the previously characterized spectrum of MDF/bH-L-H heterodimers in vitro, MCK-L type E-boxes probably bind qualitatively different factors in vivo.

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Documento generato il 24/09/20 alle ore 23:39:24