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Titolo:
DYNAMIC OR STABLE INTERACTIONS OF INFLUENZA HEMAGGLUTININ MUTANTS WITH COATED PITS - DEPENDENCE ON THE INTERNALIZATION SIGNAL BUT NOT ON AGGREGATION
Autore:
FIRE E; GUTMAN O; ROTH MG; HENIS YI;
Indirizzi:
TEL AVIV UNIV,GEORGE S WISE FAC LIFE SCI,DEPT BIOCHEM IL-69978 TEL AVIV ISRAEL TEL AVIV UNIV,GEORGE S WISE FAC LIFE SCI,DEPT BIOCHEM IL-69978 TEL AVIV ISRAEL UNIV TEXAS,SW MED CTR DALLAS TX 75235
Titolo Testata:
The Journal of biological chemistry
fascicolo: 36, volume: 270, anno: 1995,
pagine: 21075 - 21081
SICI:
0021-9258(1995)270:36<21075:DOSIOI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE PHOTOBLEACHING RECOVERY; RECEPTOR-MEDIATED ENDOCYTOSIS; MEMBRANE-PROTEIN TRAFFICKING; LYSOSOMAL ACID-PHOSPHATASE; LOW-DENSITY LIPOPROTEIN; CYTOPLASMIC DOMAIN; VIRUS HEMAGGLUTININ; ASIALOGLYCOPROTEIN RECEPTOR; LATERAL MOBILITY; INVITRO BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
E. Fire et al., "DYNAMIC OR STABLE INTERACTIONS OF INFLUENZA HEMAGGLUTININ MUTANTS WITH COATED PITS - DEPENDENCE ON THE INTERNALIZATION SIGNAL BUT NOT ON AGGREGATION", The Journal of biological chemistry, 270(36), 1995, pp. 21075-21081

Abstract

Measurements of the lateral mobility of native and mutated membrane proteins, combined with treatments that alter clathrin lattice structure, are capable of characterizing their interactions with coated pits in live cells (Fire, E., Zwart, D. E., Roth, M. G., and Henis, Y. I. (1991) J. Cell Biol. 115, 1585-1594). To explore the dependence of theseinteractions on the internalization signal and the aggregation state of the protein, we have extended this approach to investigate the interactions between coated pits and several influenza hemagglutinin (HA) mutants, which differ in the internalization signals in their short cytoplasmic tails. The lack of internalization signals in the trimeric wild-type HA enables a direct comparison between specific internalization signals introduced singly in each mutant. We have selected for these studies HA mutants that showed different internalization rates and varied in their tendency to aggregate into complexes larger than trimers. Our results indicate that the mode of interaction with coated pits (transient association-dissociation versus stable entrapment) depends on the internalization signal and affects the internalization efficiency. Mutants that contain a strong internalization signal and undergo fast endocytosis were entrapped in coated pits for the entire duration of the lateral mobility measurement, suggesting stable association with (slow dissociation from) coated pits. A mutant with a suboptimal internalization signal, which was internalized 10-fold slower, exhibited transient interactions with coated pits. Both types of interactions disappeared or were significantly reduced upon disruption of the clathrin lattices under hypertonic conditions, and were modulated following the ''freezing'' of coated pits by cytosol acidification. Unlike the dependence on the cytoplasmic internalization signal, the interactions with coated pits did not depend on the aggregation state (measured by sucrose gradient centrifugation after solubilization in n-octylglucoside) of the mutants.

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Documento generato il 22/01/20 alle ore 12:42:05