Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
POLAROGRAPHIC NEEDLE ELECTRODE MEASUREMENTS OF OXYGEN IN RAT PROSTATECARCINOMAS - ACCURACY AND REPRODUCIBILITY
Autore:
YEH KA; BIADE S; LANCIANO RM; BROWN DQ; FENNING MC; BABB JS; HANKS GE; CHAPMAN JD;
Indirizzi:
FOX CHASE CANC CTR,DEPT RADIAT ONCOL,7701 BURHOLME AVE PHILADELPHIA PA 19111 FOX CHASE CANC CTR,DEPT RADIAT ONCOL PHILADELPHIA PA 19111 FOX CHASE CANC CTR,DEPT SURG ONCOL PHILADELPHIA PA 19111 FOX CHASE CANC CTR,DEPT BIOSTAT PHILADELPHIA PA 19111
Titolo Testata:
International journal of radiation oncology, biology, physics
fascicolo: 1, volume: 33, anno: 1995,
pagine: 111 - 118
SICI:
0360-3016(1995)33:1<111:PNEMOO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
O2 TENSION MEASUREMENTS; PHOTODYNAMIC THERAPY; UTERINE CERVIX; HYPOXIC CELLS; TUMORS;
Keywords:
P0(2) HISTOGRAPH; TUMOR OXYGENATION; HYPOXIA; OXYGEN ELECTRODE; PROSTATE CARCINOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
K.A. Yeh et al., "POLAROGRAPHIC NEEDLE ELECTRODE MEASUREMENTS OF OXYGEN IN RAT PROSTATECARCINOMAS - ACCURACY AND REPRODUCIBILITY", International journal of radiation oncology, biology, physics, 33(1), 1995, pp. 111-118

Abstract

Purpose: The oxygenation status of tumors may be important for predicting tumor response to therapy. Previous studies with the (a)naplastic(R3327-AT) and well-differentiated (R3327-H) Dunning rat prostate tumors using indirect assays of tumor oxygenation indicated the relative hypoxic and radioresistant nature of the anaplastic tumor. We now report direct measurements of oxygen in these tumors made with the pO(2) histograph to determine: (a) whether a significant difference in oxygenation status could be detected between them; (b) whether sequential measurements on the same tumor gave similar values; and (c) whether tumor oxygenation correlated with tumor volume. Methods and Materials: R3327-AT and R3327-H tumors were grown in Fischer X Copenhagen rats to volumes of 1.0-7.0 cm(3). Electrode measurements (100-200) were made in tumors in anesthetized animal along two parallel tracks. Repeat measurements were made at 1-5 days along different parallel tracks. Oxygen partial pressures of muscle tissue were measured and served as a normaltissue control. Statistical analyses were applied to determine whether tumor oxygen levels were different between the two tumor histologies, whether sequential measurements in the same tumor were reproducible,and whether tumor oxygenation correlated with tumor volume. Results: The average median pO(2) of the well-differentiated (n = 15) and the anaplastic (n = 15) tumors was 6.0 mmHg (SE +/- 1.3) and 2.2 mmHg (SE +/- 0.3), respectively. The average median pO(2) of normal rat muscle (n = 15) was 23.6 mmHg (SE +/- 2.0). These values represent highly significant differences in oxygen concentration between the two tumors andrat muscle. The differences in average mean pO(2) values were also highly significant. Repeat measurements in the same tumors on different days gave average median values of 4.7 and 2.2 mmHg in the R3327-H (n = 15) and R3327-AT (n = 15) tumors, respectively. For these repeat measurements, median pO(2) values decreased in 15 and increased in 15 tumors, and were not significantly different from the first measurements. The average differences observed in median pO(2) were 37% (SE +/- 7) and 58% (SE +/- 10) for the R3327-H and R3327-AT tumors, respectively. No significant correlation was observed between pO(2) levels and the tumor volumes investigated in this study. Conclusions: The median pO(2) values of the anaplastic I)Dunning tumors were significantly Lower than those of the well-differentiated tumors (p < 0.001). Oxygen levelsin both tumors were significantly lower than those measured in normalrat muscle (p < 0.00005). Repeat measurements of median pO(2) in the same tumors were not significantly different for either tumor model (p> 0.5). The changes observed in pO(2) distributions within individualtumors from day to day may indicate true dynamics of its oxygenation status and/or the limits of electrode measurements, by sampling along only two insertion sites. The electrode measurements of pO(2) in thesetumor models are reproducible and confirm previously detected oxygenation differences between the anaplastic and well-differentiated tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:37:23