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Titolo:
LOW-TEMPERATURE AND FERTILIZATION-INDUCED CA2+ CHANGES IN RAT EGGS
Autore:
BENYOSEF D; ORON Y; SHALGI R;
Indirizzi:
TEL AVIV UNIV,SACKLER FAC MED,DEPT EMBRYOL IL-69978 TEL AVIV ISRAEL TEL AVIV UNIV,SACKLER FAC MED,DEPT EMBRYOL IL-69978 TEL AVIV ISRAEL TEL AVIV UNIV,SACKLER FAC MED,DEPT PHYSIOL & PHARMACOL RAMAT AVIV ISRAEL
Titolo Testata:
Molecular reproduction and development
fascicolo: 1, volume: 42, anno: 1995,
pagine: 122 - 129
SICI:
1040-452X(1995)42:1<122:LAFCCI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPETITIVE CALCIUM TRANSIENTS; MOUSE OOCYTE ACTIVATION; LIPID PHASE-TRANSITIONS; METAPHASE-II ARREST; HAMSTER EGGS; INOSITOL 1,4,5-TRISPHOSPHATE; ZONA-PELLUCIDA; ELECTRICAL-STIMULATION; RABBIT OOCYTES; IN-VITRO;
Keywords:
COOLING; [CA2+](I) TRANSIENTS; ACTIVATION; FERTILIZATION; EGG; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
D. Benyosef et al., "LOW-TEMPERATURE AND FERTILIZATION-INDUCED CA2+ CHANGES IN RAT EGGS", Molecular reproduction and development, 42(1), 1995, pp. 122-129

Abstract

In mammalian eggs, activation by sperm that leads to resumption of meiosis is characterized by an explosive transient increase in intracellular calcium ion concentration ([Ca2+](i)), followed by [Ca2+](i) oscillations. In addition to the spermatozoon, Various treatments can induce parthenogenetic activation, accompanied by an elevation of [Ca2+](i). It has been reported that cooling can induce egg activation, yet the mechanism of this phenomenon has not been elucidated. in the presentstudy we followed changes in egg [Ca2(+)]i (measured by Fura-2 fluorescence ratio imaging) during activation by cooling, using conditions that ensure a low rate of spontaneous activation. Our present findings demonstrate that cooling induces egg activation as manifested by [Ca2+](i) transient(s) and second polar body extrusion. Seventy-eight of 104 eggs responded to cooling with increased [Ca2+](i). Thirty-five percent of the responding eggs displayed a single [Ca2+](i) transient, while 65% exhibited at least two [Ca2+](i) transients within the time window of the experiment (30-40 min). Twenty-two percent of these eggs displayed high-frequency oscillations (intervals of 3.5-5.9 min). In these eggs, the overall pattern of calcium dynamics was similar to that observed in eggs activated by sperm, as judged by the transient's intervals, duration, and a gradual increase in the amplitude of successive transients. The amplitudes of [Ca2+](i) transients, however, were 2-3 times lower. We propose that cooling affects [Ca2+](i) homeostasis to produce fertilization-like changes in [Ca2+](i), possibly associated with parthenogenetic activation. Moreover, great care should be exercised to prevent temperature changes during egg handling. (C) 1995 Wiiey-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 08:44:20