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Titolo:
ORGANELLAR CLUSTERS FORMED BY MITOCHONDRIAL-ROUGH ENDOPLASMIC-RETICULUM ASSOCIATIONS - AN ORDERED ARRANGEMENT OF MITOCHONDRIA IN HEPATOCYTES
Autore:
CASCARANO J; CHAMBERS PA; SCHWARTZ E; POORKAJ P; GONDO RE;
Indirizzi:
UNIV CALIF LOS ANGELES,DEPT BIOL LOS ANGELES CA 90024
Titolo Testata:
Hepatology
fascicolo: 3, volume: 22, anno: 1995,
pagine: 837 - 846
SICI:
0270-9139(1995)22:3<837:OCFBME>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-LIVER; DIGITONIN; MEMBRANES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
J. Cascarano et al., "ORGANELLAR CLUSTERS FORMED BY MITOCHONDRIAL-ROUGH ENDOPLASMIC-RETICULUM ASSOCIATIONS - AN ORDERED ARRANGEMENT OF MITOCHONDRIA IN HEPATOCYTES", Hepatology, 22(3), 1995, pp. 837-846

Abstract

Our objective was to determine if mitochondrial-rough endoplasmic reticulum (mt-RER) associations provide for an ordered arrangement of mitochondria in the cell. If such an ordered arrangement exists, it mightbe manifested by grouping of mitochondria according to size and biochemical properties. Liver homogenate was subjected to rate zonal centrifugation for fractionating mitochondrial clusters, These clusters werethen examined for morphological and biochemical characteristics. Scanning electron microscopy (SEM) showed that (1) mitochondria were held together in clusters by rough endoplasmic reticulum, (2) clusters consisted of mitochondria of comparable size, and (3) a 45-fold differencein average mitochondrial volume existed between the organelles of thefastest and slowest sedimenting clusters. Transmission electron microscopy (TEM) affirmed that all of the organellar clusters examined weremitochondria associated with rough endoplasmic reticulum. Cytochrome oxidase and mitochondrial DNA were found to be proportional to mitochondrial volume, indicating that these components were synthesized in proportion to increases in volume. Conversely, succinic dehydrogenase and ornithine carbamoyl transferase were increased disproportionately (2.9-fold and six-fold, respectively) with increase in mitochondrial volume, It is evident from this biochemical heterogeneity that clusters composed of larger mitochondria differ functionally from clusters of smaller mitochondria, The size ordered arrangement suggests that this organization is in some way related to the biogenesis of hepatocyte mitochondria, It is also conjectured that the biochemical heterogeneity isa consequence of addition of selected proteins (e.g., succinic dehydrogenase and carbamoyl transferase) to mitochondria in a developmental process as they mature into larger organelles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 07:52:18