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Titolo:
INTERACTION OF PORPHYROMONAS-GINGIVALIS WITH TRANSFERRIN
Autore:
TAZAKI K; INOSHITA E; AMANO A; HANIOKA T; TAMAGAWA H; SHIZUKUISHI S;
Indirizzi:
OSAKA UNIV,FAC DENT,DEPT PREVENT DENT,1-8 YAMADAOKA SUITA OSAKA 565 JAPAN OSAKA UNIV,FAC DENT,DEPT PREVENT DENT SUITA OSAKA 565 JAPAN
Titolo Testata:
FEMS microbiology letters
fascicolo: 2, volume: 131, anno: 1995,
pagine: 161 - 166
SICI:
0378-1097(1995)131:2<161:IOPWT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACTOFERRIN-BINDING PROTEINS; BACTEROIDES-GINGIVALIS; HEMIN-BINDING; NEISSERIA-MENINGITIDIS; IRON; IDENTIFICATION; RECEPTOR; W50; LIMITATION; VIRULENCE;
Keywords:
PORPHYROMONAS GINGIVALIS; TRANSFERRIN BINDING; IRON; GROWTH LIMITATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
K. Tazaki et al., "INTERACTION OF PORPHYROMONAS-GINGIVALIS WITH TRANSFERRIN", FEMS microbiology letters, 131(2), 1995, pp. 161-166

Abstract

In this study, we characterized the binding of transferrin to Porphyromonas gingivalis using a classical receptor-binding assay, and examined the relationship between the binding and availability of transferrin for the growth of P. gingivalis. The binding of I-125-labeled human transferrin to P. gingivalis occurred rapidly, reversibly and specifically. Scatchard analysis yielded a K-d of 1.37 +/- 0.16 mu M and an apparent number of 1.13 +/- 0.26 x 10(5) receptors per cell. The bindingof transferrin was much increased when organisms were grown in iron-limited conditions. Among the species of black-pigmented anaerobic rods, those strains of P. gingivalis which had high transferrin-binding activity exhibited unrestricted growth following the addition of transferrin to the hemin-free culture medium. On the other hand, the presenceof transferrin in the culture medium did not support unrestricted growth of organisms that had low transferrin-binding activity. These results suggest that the binding of transferrin to P. gingivalis cells maybe a preliminary step in iron acquisition, which allows them to survive in the healthy periodontal environment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:47:09