Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
FACTORS INFLUENCING THE EFFICIENCY OF CATIONIC LIPOSOME-MEDIATED INTRAVENOUS GENE DELIVERY
Autore:
LIU Y; MOUNKES LC; LIGGITT HD; BROWN CS; SOLODIN I; HEATH TD; DEBS RJ;
Indirizzi:
CALIF PACIFIC MED RES INST,2330 CLAY ST SAN FRANCISCO CA 94115 CALIF PACIFIC MED RES INST SAN FRANCISCO CA 94115 UNIV WASHINGTON,DEPT COMPARAT MED SEATTLE WA 98195 UNIV WISCONSIN,SCH PHARM MADISON WI 53706
Titolo Testata:
Nature biotechnology
fascicolo: 2, volume: 15, anno: 1997,
pagine: 167 - 173
SICI:
1087-0156(1997)15:2<167:FITEOC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; CYSTIC-FIBROSIS; DNA-TRANSFECTION; CELLS; MICE; EXPRESSION; THERAPY; CHOLESTEROL; INVIVO; SERUM;
Keywords:
GENE TRANSFER; CATIONIC LIPOSOMES; GENE THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
Y. Liu et al., "FACTORS INFLUENCING THE EFFICIENCY OF CATIONIC LIPOSOME-MEDIATED INTRAVENOUS GENE DELIVERY", Nature biotechnology, 15(2), 1997, pp. 167-173

Abstract

We have characterized the relationships between the design of cationic liposomes as a gene transfer vehicle, their resulting biodistribution and processing in animals, and the level and sites of gene expression they produce. By redesigning conventional cationic liposomes, incorporating cholesterol (chol) as the neutral lipid and preparing them as multilamellar vesicles (MLV), we increased the efficiency of cationic liposome:DNA complex (CLDC)-mediated gene delivery, Expression of the luciferase gene increased up to 1,740-fold and of the human granulocyte-colony stimulating factor (hG-CSF) gene up to 569-fold due to prolonged circulation time of injected CLDC, and increased uptake and retention in tissues. The level of gene expression per microgram of DNA taken up per tissue was 1,000-fold higher in lung than in liver, indicating that in addition to issues of delivery and retention of injected DNA, tissue-specific host factors also play a central role in determiningthe efficiency of expression. Vascular endothelial cells, monocytes, and macrophages are the cell types most commonly transfected by intravenous injection of CLDC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:57:51