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Titolo:
MODIFIED ANTISENSE OLIGODEOXYNUCLEOTIDES AGAINST THE SPLICE ACCEPTOR SITE OF TOT DO NOT INHIBIT IN-VITRO HEMATOPOIETIC COLONY GROWTH IN HIV-POSITIVE PATIENTS
Autore:
GEISSLER RG; MUTH J; MAURER A; MENTZEL U; MAG M; ENGELS JW; HOELZER D; GANSER A;
Indirizzi:
UNIV FRANKFURT,DEPT HEMATOL,THEODOR STERN KAI 7 D-60590 FRANKFURT GERMANY UNIV FRANKFURT,INST ORGAN CHEM D-60439 FRANKFURT GERMANY
Titolo Testata:
Annals of hematology
fascicolo: 2, volume: 71, anno: 1995,
pagine: 89 - 95
SICI:
0939-5555(1995)71:2<89:MAOATS>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; AIDS-RELATED COMPLEX; CHRONICALLY INFECTED-CELLS; PLACEBO-CONTROLLED TRIAL; PROGENITOR CELLS; REVERSE-TRANSCRIPTASE; TAT PROTEIN; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES; AZIDOTHYMIDINE AZT; INVITRO GROWTH;
Keywords:
ANTISENSE OLIGONUCLEOTIDES; TAT GENE; HEMATOPOIETIC COLONY GROWTH; ACQUIRED IMMUNODEFICIENCY SYNDROME; HUMAN IMMUNODEFICIENCY VIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
R.G. Geissler et al., "MODIFIED ANTISENSE OLIGODEOXYNUCLEOTIDES AGAINST THE SPLICE ACCEPTOR SITE OF TOT DO NOT INHIBIT IN-VITRO HEMATOPOIETIC COLONY GROWTH IN HIV-POSITIVE PATIENTS", Annals of hematology, 71(2), 1995, pp. 89-95

Abstract

The hematopoietic failure in the majority of patients with progressive HIV infection is further aggravated by virustatic agents like azidothymidine. As an alternative therapeutic attempt, three derivatives of an antisense oligodeoxynucleotide (ODN) against the splice acceptor site of the tat gene have been shown to inhibit HIV replication in vitro. This study was aimed at examining whether these agents are toxic to the hematopoietic progenitor cells. To this end, bone marrow cells from HIV-positive and healthy persons were depleted from adherent cells to eliminate fibroblasts. In further experiments, the cells were additionally enriched for CD34-positive hematopoietic progenitor cells or were depleted from delta TCS-1-positive T lymphocytes. At concentrationsof 1.25-10 mu M, the three antisense ODN did not inhibit any erythrocyte or granulocyte-monocyte colony growth from CD34-positive cells, either from the HIV-positive or from the HIV-negative cohort. In contrast to azidothymidine, which served as inhibitory control, a significantincrease of colony growth was seen after depletion of fibroblasts, ofdelta TCS-1-positive cells, or without cell separation. In conclusion, the three oligodeoxynucleotides do not exert any hematotoxic effect but do increase colony formation from low-density bone marrow cells invitro and could therefore be useful in future clinical studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 08:08:07