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Titolo:
QUANTITATION OF PEPTIDE-HORMONE IN SINGLE CULTURED SECRETORY NEURONS OF THE CRAB, CARDISOMA CARNIFEX
Autore:
KELLER R; GRAU S; COOKE IM;
Indirizzi:
UNIV HAWAII,BEKESY LAB NEUROBIOL HONOLULU HI 96822 UNIV HAWAII,BEKESY LAB NEUROBIOL HONOLULU HI 96822 UNIV HAWAII,DEPT ZOOL HONOLULU HI 96822 UNIV BONN,INST ZOOPHYSIOL D-53115 BONN GERMANY
Titolo Testata:
Cell and tissue research
fascicolo: 3, volume: 281, anno: 1995,
pagine: 525 - 532
SICI:
0302-766X(1995)281:3<525:QOPISC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
SINUS GLAND; HYPERGLYCEMIC HORMONE; CARCINUS-MAENAS; INHIBITING HORMONE; SHORE CRAB; NEUROPEPTIDES; IDENTIFICATION; CRUSTACEANS; EYESTALK;
Keywords:
NEUROENDOCRINE CELLS; NEURONAL REGENERATION; DEFINED CULTURE; NEUROSECRETION, PEPTIDERGIC; CRUSTACEAN HYPERGLYCEMIC HORMONE; ELISA; X-ORGAN; SINUS GLAND; CARDISOMA CARNIFEX (CRUSTACEA);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
R. Keller et al., "QUANTITATION OF PEPTIDE-HORMONE IN SINGLE CULTURED SECRETORY NEURONS OF THE CRAB, CARDISOMA CARNIFEX", Cell and tissue research, 281(3), 1995, pp. 525-532

Abstract

The content of crustacean hyperglycemic hormone (CHH) in single cultured neurons of the crab Cardisoma carnifex was determined by a sensitive enzyme-linked immunosorbent assay (ELISA), using purified CHH (1-50pg) of the crab Carcinus maenas as stan dard. The somata were dissociated from the group of similar to 150 peptidergic neurons that form the X-organ - sinus gland neuroendocrine system. As previously reported,the neurons show immediate regenerative outgrowth in defined culture conditions, and develop, generally, into one of two morphological types: cells that produce broad, lamelliform growth cones (veils), and others that are characterized by branching of neurites. In this study, all but one of 64 veiling cells taken after various times in culture up to 12 days contained CHH. They could be readily categorized as having ''high'' (>33 pg; mean 86+/-5, S.E., n=47) or ''low'' (less than or equal to 33pg; mean 22+/-2.5; n=17) Carcinus CHH equivalents. Thus, CHH is associated with neurons showing veiling outgrowth, but veiling neurons with low CHH form a distinct, but not morphologically distinguishable group. They may contain an isoform of CHH with limited cross-reactivity. In 24 branching neurons assayed, Carcinus CHH equivalents averaged 7.2+/-2 pg. This figure includes 14 neurons in which CHH was undetectable, and one that had 40 pg of Carcinus CHH equivalents. There wasno significant change of the hormone content in cells of either type during 6 days of culturing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:23:23