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Titolo:
ASSOCIATION OF HSP60-LIKE PROTEINS WITH THE L-SYSTEM AMINO-ACID TRANSPORTER
Autore:
WOODLOCK TJ; CHEN XX; YOUNG DA; BETHLENDY G; LICHTMAN MA; SEGEL GB;
Indirizzi:
UNIV ROCHESTER,SCH MED,DEPT PEDIAT,601 ELMWOOD AVE,BOX 777 ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED,DEPT PEDIAT ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED,DEPT MED ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED,DEPT BIOPHYS ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED,ST MARYS HOSP,CTR CANC ROCHESTER NY 14642
Titolo Testata:
Archives of biochemistry and biophysics
fascicolo: 1, volume: 338, anno: 1997,
pagine: 50 - 56
SICI:
0003-9861(1997)338:1<50:AOHPWT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC LYMPHOCYTIC-LEUKEMIA; HEAT-SHOCK PROTEIN-60; GLUTAMATE TRANSPORTER; SEQUENCE SIMILARITY; EXPRESSION CLONING; RAT-KIDNEY; MEMBRANE; CELLS; NA+; IDENTIFICATION;
Keywords:
L-SYSTEM AMINO ACID TRANSPORT; HEAT SHOCK PROTEIN 60; CHRONIC LYMPHOCYTIC LEUKEMIA; PHORBOL ESTER; CELL MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
T.J. Woodlock et al., "ASSOCIATION OF HSP60-LIKE PROTEINS WITH THE L-SYSTEM AMINO-ACID TRANSPORTER", Archives of biochemistry and biophysics, 338(1), 1997, pp. 50-56

Abstract

The carrier protein(s) responsible for mammalian L-system amino acid transport has not been identified, Chronic lymphocytic leukemia (CLL) B-lymphocytes have markedly diminished L-system transport which is restored after treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). Six candidate L-system related plasma membrane proteins were identified in TPA-treated CLL-cells using an L-system photoprobe and ultra-high-resolution two-dimensional gel electrophoresis, In the current studies, two candidate L system related proteins were obtained from preparative giant two dimensional gels, and the amino acid sequences of peptide fragments from these proteins were determined by Edman degradation,These proteins, forming a doublet on the gels at an apparent size of 40 kDa and pI 5.85, had sequence homology to the mitochondrial heat shock protein 60 (hsp60). The presence of these proteins in CLL lymphocyte plasma membranes was confirmed by immunoblotting with antibodies tohsp6O. These observations indicate that an hsp60 homologue is associated with the L-system amino acid transporter. (C) 1997 Academic Press.

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Documento generato il 28/01/21 alle ore 07:47:55