Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IMMUNOMODULATORY ACTIVITIES OF EXTRACELLULAR METALLOTHIONEIN .2. EFFECTS ON MACROPHAGE FUNCTIONS
Autore:
YOUN J; BORGHESI LA; OLSON EA; LYNES MA;
Indirizzi:
UNIV CONNECTICUT,DEPT MOLEC & CELL BIOL,U-125,LSA 207 STORRS CT 06269 UNIV CONNECTICUT,DEPT MOLEC & CELL BIOL STORRS CT 06269
Titolo Testata:
Journal of toxicology and environmental health
fascicolo: 4, volume: 45, anno: 1995,
pagine: 397 - 413
SICI:
0098-4108(1995)45:4<397:IAOEM.>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-LIVER; CADMIUM-METALLOTHIONEIN; GENE-EXPRESSION; ZINC-METABOLISM; FREE-RADICALS; HEAVY-METALS; CELLS; NEPHROTOXICITY; NEUTROPHILS; NEPHROPATHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
J. Youn et al., "IMMUNOMODULATORY ACTIVITIES OF EXTRACELLULAR METALLOTHIONEIN .2. EFFECTS ON MACROPHAGE FUNCTIONS", Journal of toxicology and environmental health, 45(4), 1995, pp. 397-413

Abstract

Metallothionein (MT) is a thiol-rich protein that is rapidly induced by exposure to heavy metal cations. We have previously demonstrated that exogenous MT stimulates murine splenocytes to proliferate, but inhibits humoral responses to antigen. These observations suggest that metallothionein released from cells has a complex role in heavy metal-mediated immune dysfunction. Here we examine one possible mechanism by which MT mediates suppression of humoral immunity. Exposure of macrophages to 20 mu M MT did nor affect their ability to engulf opsonized sheep erythrocytes, but in the presence of 20 mu M MT, peritoneal macrophages were stimulated to produce increased levels of oxygen radicals. These results correlated with observations that while macrophage phagocytosis of opsonized Candida albicans was unaltered by the presence of exogenous MT, killing of the engulfed yeast cells was dramatically enhanced by 20 mu M MT. Amounts of free cadmium and zinc equimolar to thatadded as Zn,Cd-MT had no effect on candidacidal activity. MT was alsofound to significantly decrease lymphocyte proliferation mediated by macrophage activity. Biotinylated MT (MT-b) bound specifically to the plasma membranes of these macrophages, suggesting that membrane-associated molecules of the macrophage may transduce a signal mediated by MTbinding. These results demonstrate that macrophages are a sensitive target for MT-mediated immunomodulation and that some of the consequences of the MT interaction with macrophages may be alterations in the capacity to produce an effective immune response and increased extracellular exposure to damaging free radicals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:34:42