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Titolo:
DETERMINATION OF TOLFENAMIC ACID IN HUMAN PLASMA BY HPLM
Autore:
NIOPAS I; GEORGARAKIS M;
Indirizzi:
ARISTOTELIAN UNIV THESSALONIKI,DEPT PHARM GR-54006 THESSALONIKI GREECE
Titolo Testata:
Journal of liquid chromatography
fascicolo: 13, volume: 18, anno: 1995,
pagine: 2675 - 2682
SICI:
0148-3919(1995)18:13<2675:DOTAIH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHY; PHARMACOKINETICS; ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
I. Niopas e M. Georgarakis, "DETERMINATION OF TOLFENAMIC ACID IN HUMAN PLASMA BY HPLM", Journal of liquid chromatography, 18(13), 1995, pp. 2675-2682

Abstract

Tolfenamic acid is a potent prostaglandin synthetase inhibitor used clinically as non-steroidal anti-inflammatory and analgesic-antipyreticagent. A simple, sensitive, accurate, and precise reverse phase high performance Liquid chromatographic method has been developed and validated for the quantitative determination of tolfenamic acid in small volumes of human plasma. The chromatographic separation of tolfenamic acid and the internal standard (phenylbutazone) was performed on a reversed phase, 5-mu m C18 column (250 x 4 mm) using acetonitrile-10 mM phosphoric acid (60:40, v/v) as mobile phase with a flow rate of L1 ml/min and the chromatographic peaks were detected at 280 nm. Plasma was deproteinized with acetonitrile, the supernatant fraction was evaporatedto dryness and the resulting residue was reconstituted in the mobile phase and injected into the HPLC system. Calibration curves were linear in the range 0.2-5.0 mu g/ml with a squared correlation coefficient (r(2)) of 0.999 or better and the detection limit was 50 ng/ml for 100-mu l plasma samples. The method was not interfered with by other endogenous compounds or metabolites and one assay can be completed in 12 min. The within-day and between-day assay variation for three differentconcentrations was found to be less than 6% and the accuracy was nearly 100%.

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Documento generato il 29/11/20 alle ore 16:14:31