Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECTS OF GLUCOSE AND OTHER ENERGY SUBSTRATES ON THE HYPERACTIVATED MOTILITY OF MACAQUE SPERM AND THE ZONA-PELLUCIDA-INDUCED ACROSOME REACTION
Autore:
VANDEVOORT CA; OVERSTREET JW;
Indirizzi:
LAWRENCE LIVERMORE NATL LAB,CALIF REG PRIMATE RES CTR DAVIS CA 95616 UNIV CALIF DAVIS,SCH MED,DEPT OBSTET & GYNECOL,DIV REPROD BIOL & MED DAVIS CA 95616
Titolo Testata:
Journal of andrology
fascicolo: 4, volume: 16, anno: 1995,
pagine: 327 - 333
SICI:
0196-3635(1995)16:4<327:EOGAOE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-SPERMATOZOA; CAPACITATION; INVITRO; FERTILIZATION; PENETRATION; CYNOMOLGUS; CALCIUM;
Keywords:
MACACA FASCICULARIS; SEMEN; HYPERACTIVATION; ACROSOME REACTION; LACTATE; PYRUVATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Vandevoort e J.W. Overstreet, "EFFECTS OF GLUCOSE AND OTHER ENERGY SUBSTRATES ON THE HYPERACTIVATED MOTILITY OF MACAQUE SPERM AND THE ZONA-PELLUCIDA-INDUCED ACROSOME REACTION", Journal of andrology, 16(4), 1995, pp. 327-333

Abstract

Energy sources in sperm capacitation media have various effects on mammalian sperm and are required for stimulation of hyperactivated motility and/or acrosome reactions in some species. The present experimentswere performed to investigate the energy substrate requirement for these two functions of macaque sperm. Semen from six cynomolgus macaqueswas washed through 60% Percoll, resuspended, and washed with Biggers,Whitten, and Whittingham media. In one set of experiments, sperm wereincubated in the complete capacitation medium or in medium without glucose. In another set of experiments, the complete medium was used forcomparison with medium containing no energy substrates. The absence of glucose did not affect survival of sperm during a 6-hour incubation period; however, removal of all energy substrates resulted in a decrease in percent motility by 3 hours. Sperm were incubated for 1 hour prior to evaluation of sperm motility by computer-aided sperm analysis and sperm-zona binding experiments. During the last 30 minutes of incubation, half of the aliquots of sperm suspensions were treated with activators (ACT; caffeine and dbcAMP, 1 mM each). As previously reported, when sperm were incubated in complete medium and treated with ACT there were changes in sperm movements that are consistent with hyperactivation. Similar or greater changes were observed in sperm that were incubated prior to treatment with ACT in glucose-free medium or in medium without any energy substrates. Whether sperm were incubated in complexmedium or in glucose-free medium, sperm binding to zonae was enhancedwhen the sperm were treated with ACT. The absence of glucose in the incubation medium had no deleterious effect on the zona-induced acrosome reaction, which could be demonstrated whether or not sperm were treated with ACT. Sperm incubated in medium without energy substrates werealso capable of binding to the zona pellucida and of undergoing the acrosome reaction following treatment with ACT. However, the percentageof acrosome-reacted sperm on the zonae was significantly lower when sperm were incubated in medium without any substrates. These results indicate that macaque sperm, like human sperm, do not require energy substrates in incubation media in order to undergo capacitation in vitro,although energy substrates may be necessary for optimal capacitation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 19:09:11