Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DEVELOPMENTAL-CHANGES IN THE DISTRIBUTION OF THE ENDOPLASMIC-RETICULUM AND INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS AND THE SPATIAL PATTERN OF CA2+ RELEASE DURING MATURATION OF HAMSTER OOCYTES
Autore:
SHIRAISHI K; OKADA A; SHIRAKAWA H; NAKANISHI S; MIKOSHIBA K; MIYAZAKI S;
Indirizzi:
TOKYO WOMENS MED COLL,DEPT PHYSIOL,SHINJUKU KU TOKYO 162 JAPAN TOKYO WOMENS MED COLL,DEPT PHYSIOL,SHINJUKU KU TOKYO 162 JAPAN TOHO UNIV,SCH MED,DEPT ANAT,OHTA KU TOKYO 143 JAPAN JT INC,CENT PHARMACEUT RES INST,PHARMACEUT BASIC RES LABS,KAWAZAWA KUYOKOHAMA KANAGAWA 236 JAPAN UNIV TOKYO,INST MED SCI,DEPT MOLEC NEUROBIOL,MINATO KU TOKYO 108 JAPAN
Titolo Testata:
Developmental biology
fascicolo: 2, volume: 170, anno: 1995,
pagine: 594 - 606
SICI:
0012-1606(1995)170:2<594:DITDOT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL GRANULE EXOCYTOSIS; XENOPUS-LAEVIS OOCYTES; MEIOTIC MATURATION; MAMMALIAN EGGS; ZONA-PELLUCIDA; SEA-URCHIN; CYTOSKELETAL SHEETS; INVITRO MATURATION; MOUSE OOCYTES; FERTILIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
K. Shiraishi et al., "DEVELOPMENTAL-CHANGES IN THE DISTRIBUTION OF THE ENDOPLASMIC-RETICULUM AND INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS AND THE SPATIAL PATTERN OF CA2+ RELEASE DURING MATURATION OF HAMSTER OOCYTES", Developmental biology, 170(2), 1995, pp. 594-606

Abstract

During maturation of hamster oocytes, the distribution of the endoplasmic reticulum (ER) and inositol 1,4,5-trisphosphate receptors (InsP(3)Rs) was found to change dramatically, as observed using confocal microscopy with DiI and electron microscopy for the ER and immunohistochemistry for InsP(3)Rs. In immature oocytes at the germinal vesicle (GV) stage, ER and InsP(3)Rs were located predominantly in several large masses near the surface and also in the perinuclear region near the surface. In contrast, fine ER networks and few-density InsP(3)Rs were present in the inner cytoplasm. The ER appeared to be formed as vesicles from annulate lamellae (AL) in the subcortical area. Rises in Ca2+ concentration occurred in the cytoplasm and the GV when immature oocytes were inseminated, but clear Ca2+ waves did not occur. Ca2+ rises began preferentially from the perinuclear region in response to low doses ofserotonin or to uniform stimulation of InsP(3)Rs with photocleavage of caged InsP(3). Serum also induced inhomogeneous Ca2+ release, shown by nonpropagating Ca2+ rises at multiple surface sites. Between the GVstage and pro-metaphase I the number and size of the surface ER masses increased, and the AL disappeared. This quantitative ER maturation was followed by a second step, spatial maturation. After prometaphase I, surface ER masses gradually dispersed to a number of much smaller ERclusters near the surface and, together with the perinuclear mass, were incorporated into thicker ER networks, resulting in a reticular pattern of the ER with small patches of InsP(3)Rs throughout the mature egg. The ER shifted to the peripheral surface with apposition to cortical granules. These developmental changes in ER Ca2+ stores may account, at least partly, for the acquisition of the ability of an egg to undergo normal fertilization. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 03:02:13