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Titolo:
IMMUNOHISTOCHEMICAL LOCALIZATION AND POSSIBLE PHYSIOLOGICAL ROLES OF TUMOR-NECROSIS-FACTOR-ALPHA IN MOUSE CUMULUS-OOCYTE COMPLEXES
Autore:
SATO E; NAKAYAMA T; KAMIO K; TAKAHASHI Y; TOYODA Y;
Indirizzi:
UNIV TOKYO,INST MED SCI,DEPT REPROD & DEV BIOL,MINATO KU,4-6-1 SHIROKANEDAI TOKYO 108 JAPAN NIHON UNIV,DEPT APPL BIOL FUJISAWA KANAGAWA 252 JAPAN
Titolo Testata:
Development, growth & differentiation
fascicolo: 4, volume: 37, anno: 1995,
pagine: 413 - 420
SICI:
0012-1592(1995)37:4<413:ILAPPR>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE-INDUCED DIFFERENTIATION; OVARIAN GRANULOSA-CELLS; PROGESTERONE PRODUCTION; CORPUS-LUTEUM; INVITRO; RAT; MACROPHAGES; EXPRESSION; FOLLICLES; GLYCOSAMINOGLYCANS;
Keywords:
CUMULUS-OOCYTE COMPLEXES; IN VITRO FERTILIZATION; MOUSE; OOCYTE MATURATION; TUMOR NECROSIS FACTOR-ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
E. Sato et al., "IMMUNOHISTOCHEMICAL LOCALIZATION AND POSSIBLE PHYSIOLOGICAL ROLES OF TUMOR-NECROSIS-FACTOR-ALPHA IN MOUSE CUMULUS-OOCYTE COMPLEXES", Development, growth & differentiation, 37(4), 1995, pp. 413-420

Abstract

Tumor necrosis factor-alpha (TNF-alpha), a cytokine which is producedby activated macrophages, has been shown to participate in the regulation of ovarian functions. In the course of our investigation on the mechanism of maturation, fertilization and degeneration of mouse oocytes, immunoreactivity to TNF-alpha was found in the cytoplasm of the cells surrounding the maturing oocytes and of granulosa cells facing the antral cavity. Immunoblot analysis with the specific antibody to TNF-alpha identified the 17 kDa Mr band in the extract of cumulusoocyte complexes. Various concentrations of TNF-alpha (mouse, recombinant) and anti TNF-alpha antiserum (polyclonal rabbit anti-mouse recombinant TNF-alpha) were then used to determine their effect on the germinal vesicle breakdown (GVBD), polar body extrusion, fertilization and fragmentation of mouse oocytes/eggs. TNF-alpha at concentrations of 10 ng/mt or less and anti-TNF-alpha antiserum at concentrations of 10% or less, had no effect on the spontaneous GVBD and polar body extrusion of mouse oocytes in culture. Mouse follicular oocytes cultured for more than 72h in modified Krebs-Ringer solution in vitro undergo spontaneous fragmentation, which is a degenerative change to form 'blastomeres' with or without nuclear fragments or chromatin. Ghost-like blastomeres were also identified in the space among fragmented 'blastomeres'. The spontaneous fragmentation of mouse follicular oocytes was suppressed in thepresence of TNF-alpha at concentrations of 1 ng/mL or greater. Anti-TNF-alpha antiserum (1%) accelerated the induction of fragmentation of oocytes cultured in vitro. The addition of anti TNF-alpha antiserum (10%) to the culture medium did not influence the fertilization rates ofthe eggs surrounded by the expanded cumulus. These results appear to indicate that the process of degeneration of mouse oocytes/eggs is modulated by TNF-alpha accumulated in the expanded cumulus during oocyte maturation.

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Documento generato il 02/07/20 alle ore 22:21:57