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Titolo:
REGULATION OF HYPOTHALAMIC AND PITUITARY CORTICOTROPIN-RELEASING HORMONE-RECEPTOR MESSENGER-RIBONUCLEIC-ACID BY ADRENALECTOMY AND GLUCOCORTICOIDS
Autore:
LUO X; KISS A; RABADANDIEHL C; AGUILERA G;
Indirizzi:
NICHHD,DEV BIOL BRANCH,SECT ENDOCRINE PHYSIOL,BLDG 10,ROOM 10N262,10 CTR DR,MSC 1862 BETHESDA MD 20892 NICHHD,DEV BIOL BRANCH,SECT ENDOCRINE PHYSIOL BETHESDA MD 20892
Titolo Testata:
Endocrinology
fascicolo: 9, volume: 136, anno: 1995,
pagine: 3877 - 3883
SICI:
0013-7227(1995)136:9<3877:ROHAPC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR CRF RECEPTORS; MEDIAN-EMINENCE; ADRENOCORTICOTROPIN SECRETION; PARAVENTRICULAR NUCLEUS; RAT-BRAIN; VASOPRESSIN; STRESS; AXIS; RESPONSES; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
X. Luo et al., "REGULATION OF HYPOTHALAMIC AND PITUITARY CORTICOTROPIN-RELEASING HORMONE-RECEPTOR MESSENGER-RIBONUCLEIC-ACID BY ADRENALECTOMY AND GLUCOCORTICOIDS", Endocrinology, 136(9), 1995, pp. 3877-3883

Abstract

The effects of adrenalectomy and glucocorticoids on the regulation ofcorticotropin-releasing hormone (CRH) receptor expression in the hypothalamic paraventricular nucleus (PVN) and pituitary were studied by in situ hybridization in the rat using a complementary RNA probe directed toward the coding region of the type 1 CRH receptor. Eighteen hoursafter adrenalectomy, CRH receptor messenger RNA (mRNA) expression in the PVN was significantly increased, whereas longer term adrenalectomy(4 and 6 days) had no effect. This transient effect of adrenalectomy was prevented by glucocorticoid replacement. In intact rats, 4 h afterimmobilization for 1 h or a single ip hypertonic saline injection, CRH receptor mRNA in the PVN markedly increased (P < 0.01), an effect that was unchanged by adrenalectomy (4 or 6 days) or dexamethasone injection (100 mu g at -14 and 50 mu g at -1 h) before stress. In the pituitary, CRH receptor mRNA levels decreased transiently after adrenalectomy (-62% after 18 h), returning to basal levels 4 or 6 days after adrenalectomy. The early decrease was prevented by glucocorticoid replacement. In intact rats, dexamethasone (100 mu g, sc) caused a significantdecrease in pituitary CRH receptor mRNA levels 2-10 h after injection, returning to basal levels after 15 h. On the other hand, dexamethasone (5-300 mu g, sc) had no effect on pituitary CRH receptor mRNA levels 18 h after injection. The data show that although stress stimulationof CRH mRNA in the PVN is glucocorticoid independent, basal levels are likely to be under dual, transcriptional and posttranscriptional, control by glucocorticoids. In the pituitary, changes in hypothalamic CRFs probably play a major role in the control of CRH receptor mRNA levels during manipulations of circulating glucocorticoids levels. In addition, the inability of long term adrenalectomy and glucocorticoid administration to modify pituitary CRH receptor mRNA levels suggests that CRH receptor down-regulation observed under these experimental conditions depends mainly on translational and posttranslational events rather than receptor mRNA levels.

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Documento generato il 28/11/20 alle ore 00:32:05