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Titolo:
CONSERVED GENE-SEQUENCES FOR SPECIES IDENTIFICATION - PCR ANALYSIS OFTHE 3'-UTR OF THE SON GENE DISTINGUISHES HUMAN AND OTHER MAMMALIAN DNAS
Autore:
SOTERIOU B; FISHER RA; KHAN IM; KESSLING AM; ARCHARD LC; BULUWELA L;
Indirizzi:
UNIV LONDON,CHARING CROSS & WESTMINSTER MED SCH,DEPT BIOCHEM,FULHAM PALACE RD LONDON W6 8RF ENGLAND UNIV LONDON,CHARING CROSS & WESTMINSTER MED SCH,DEPT BIOCHEM LONDON W6 8RF ENGLAND UNIV LONDON,CHARING CROSS & WESTMINSTER MED SCH,DEPT MED ONCOL LONDONW6 8RF ENGLAND ST MARYS HOSP,SCH MED,DEPT BIOCHEM & MOLEC GENET LONDON W2 1PG ENGLAND
Titolo Testata:
Forensic science international
fascicolo: 3, volume: 73, anno: 1995,
pagine: 171 - 181
SICI:
0379-0738(1995)73:3<171:CGFSI->2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE; PROTEIN;
Keywords:
SPECIES IDENTIFICATION; PCR; SON GENE; GENESCAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
B. Soteriou et al., "CONSERVED GENE-SEQUENCES FOR SPECIES IDENTIFICATION - PCR ANALYSIS OFTHE 3'-UTR OF THE SON GENE DISTINGUISHES HUMAN AND OTHER MAMMALIAN DNAS", Forensic science international, 73(3), 1995, pp. 171-181

Abstract

In this paper we show that the polymerase chain reaction (PCR) can beused on regions of highly conserved genes, such as the 3' untranslated region (3' UTR) of the SON gene, to identify the mammalian origin ofa sample. Using this test, we have been able to distinguish human, monkey, cat, dog, mouse and hamster DNAs. We have also determined the DNA sequence of these different PCR products, which can be used to reinforce species identification. The advantages of this test are that: (i)no prior information is required on the possible species origin of a sample, (ii) the reaction produces a single PCR product which varies in size according to the species of origin, making the test simple to interpret, and (iii) the target region of DNA amplified in these experiments is small and can easily be sequenced and sized using automated techniques. This small size has enabled us to successfully amplify thisproduct from DNA extracted from compromised material (human bone samples) and so demonstrate that the test is valuable for the characterisation of remains in which DNA is degraded.

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Documento generato il 28/11/20 alle ore 18:13:37