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Titolo:
COUPLING OF THE CLONED MU-OPIOID RECEPTOR WITH THE OMEGA-CONOTOXIN-SENSITIVE CA2-15 CELLS( CURRENT IN NG108)
Autore:
MORIKAWA H; FUKUDA K; KATO S; MORI K; HIGASHIDA H;
Indirizzi:
KYOTO UNIV HOSP,DEPT ANESTHESIA,SAKYO KU KYOTO 60601 JAPAN KANAZAWA UNIV,SCH MED,DEPT BIOPHYS KANAZAWA ISHIKAWA 920 JAPAN
Titolo Testata:
Journal of neurochemistry
fascicolo: 3, volume: 65, anno: 1995,
pagine: 1403 - 1406
SICI:
0022-3042(1995)65:3<1403:COTCMR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURONAL CALCIUM CHANNELS; GTP-BINDING PROTEIN; HIGH-THRESHOLD; INHIBITION; SUBTYPES; EXPRESSION; CYCLASE;
Keywords:
OPIOID RECEPTOR; CDNA EXPRESSION; CA2+ CHANNELS; ADENYLATE CYCLASE; NEUROBLASTOMA X GLIOMA HYBRID CELLS;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
H. Morikawa et al., "COUPLING OF THE CLONED MU-OPIOID RECEPTOR WITH THE OMEGA-CONOTOXIN-SENSITIVE CA2-15 CELLS( CURRENT IN NG108)", Journal of neurochemistry, 65(3), 1995, pp. 1403-1406

Abstract

Voltage-dependent Ca(2+-)currents were measured in NG108-15 neuroblastoma x glioma hybrid cells transformed to express the rat mu-opioid receptor by the whole-cell configuration of the patch-clamp technique with Ba2+ as charge carrier. A mu-opioid opioid receptor-selective agonist, [D-Ala(2),N-Me-Phe(4),Gly(5)-ol]-enkephalin caused significant inhibition of voltage-dependent Ca2+ currents in mu-receptor-transformed NG108-15 cells but not in nontransfected or vector-transformed controlcells. On the other hand, a delta-opioid receptor-selective agonist, [D-penicillamine(2),D-penicillamine(5)]enkephalin, induced inhibition of voltage-dependent Ca2+ currents in both control and mu-receptor-transformed cells, which is mediated by the delta-opioid receptor expressed endogenously in NG108-15 cells. The inhibition of voltage-dependentCa2+ currents induced by [D-Ala(2), N-Me-Phe(4),Gly(5)-ol]enkephalin and [D-penicillamine(2),D-penicillamine(5)]enkephalin was reduced by pretreatment of the cells with pertussis toxin or omega-conotoxin GVIA. These results indicate that the mu-opioid receptor expressed from cDNA functionally couples with omega-conotoxin-sensitive N-type Ca2+ channels through the action of pertussis toxin-sensitive G proteins in NG108-15 cells.

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Documento generato il 19/09/20 alle ore 13:44:57