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Titolo:
THE EXTRACTION OF RADIOLABELED INORGANIC SULFATE FROM BLOOD-PLASMA
Autore:
WANG Y; BARRY TN;
Indirizzi:
MASSEY UNIV,DEPT ANIM SCI PALMERSTON NORTH NEW ZEALAND MASSEY UNIV,DEPT ANIM SCI PALMERSTON NORTH NEW ZEALAND
Titolo Testata:
Journal of the Science of Food and Agriculture
fascicolo: 4, volume: 68, anno: 1995,
pagine: 417 - 420
SICI:
0022-5142(1995)68:4<417:TEORIS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Keywords:
INORGANIC SULFATE; EXTRACTION; PLASMA; ELUENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
5
Recensione:
Indirizzi per estratti:
Citazione:
Y. Wang e T.N. Barry, "THE EXTRACTION OF RADIOLABELED INORGANIC SULFATE FROM BLOOD-PLASMA", Journal of the Science of Food and Agriculture, 68(4), 1995, pp. 417-420

Abstract

A study was conducted into factors governing the efficiency of the ion exchange method for extracting S-35-labelled inorganic sulphate (SO42-) from blood plasma, using Dowex-1-X8 ion exchange resin. The study compared effects of trichloroacetic acid (TCA) strength as protein precipitant, different HCl strengths as resin eluent, sodium citrate/HCl (SC/HCl) versus HCl as eluents, and evaluated ultrafiltrated (UF) plasma upon the adsorption and recovery of added (SO42-)-S-35. Both adsorption and release of (SO42-)-S-35 from the resin were inhibited by the presence of TCA, and HCl was not as effective as 1 M SC/2 M HCl in releasing (SO42-)-S-35 adsorbed to resin. The rates of (SO42-)-S-35 adsorbed onto resin and recovered were markedly increased by using UF plasma and 1 M SC/2 M HCl as eluent, with the values being 96.3 +/- 0.11% and 91.1 +/- 0.39%, respectively, where 1 g resin was used. Therefore, the use of UF for deproteinising and 1 M SC/2 M HCl as eluent are recommended for extracting (SO42-)-S-35 from blood plasma when Dowex 1-X8 resin is used as the ion exchanger.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 18:11:07