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Titolo:
REEVALUATION OF ANTI-HTLV-I WESTERN-BLOT ASSAY USING HTLV-I AND HTLV-II SERUM PANELS
Autore:
FUJIYAMA C; FUJIYOSHI T; MATSUMOTO D; YASHIKI S; TAMASHIRO H; SONODA S;
Indirizzi:
KAGOSHIMA UNIV,FAC MED,DEPT VIROL,8-35-1 SAKURAGAOKA KAGOSHIMA 890 JAPAN KAGOSHIMA UNIV,FAC MED,DEPT VIROL KAGOSHIMA 890 JAPAN KAGOSHIMA INST PREVENT MED KAGOSHIMA JAPAN WHO,OFF RES GLOBAL PROGRAMME AIDS,DIAGNOST UNIT,CLIN RES & PROD DEV GENEVA SWITZERLAND
Titolo Testata:
Clinical and diagnostic virology
fascicolo: 2, volume: 4, anno: 1995,
pagine: 149 - 161
SICI:
0928-0197(1995)4:2<149:ROAWAU>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL LEUKEMIA; VIRUS TYPE-I; COMPLETE NUCLEOTIDE-SEQUENCE; MONONUCLEAR-CELLS; ANTIBODIES; LINE; AGGLUTINATION; CONFIRMATION; PROVIRUS; TESTS;
Keywords:
HTLV-I; HTLV-II; WESTERN BLOT CRITERION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
C. Fujiyama et al., "REEVALUATION OF ANTI-HTLV-I WESTERN-BLOT ASSAY USING HTLV-I AND HTLV-II SERUM PANELS", Clinical and diagnostic virology, 4(2), 1995, pp. 149-161

Abstract

Background: Western blot assay is accepted to be a confirmatory test of anti-HTLV-I antibodies, and several WB criteria have been proposed for confirming HTLV-I seropositivity. There is a necessity for comprehensive study on the criteria. Objectives: This study was performed to evaluate anti-HTLV-I WB kits and feasibility of the WB criteria. Studydesign: We tested 3 commercially available WB kits: PROBLOT HTLV-I, Eitest ATL-WB and HTLV BLOT 2.2, by a standard HTLV-I serum panel whichhad been established in our previous study, Sensitivity and specificity to detect each component of HTLV-I antigens were evaluated by the correlation coefficient, R-value. Specificity of the anti-HTLV-I WB kits and HTLV BLOT 2.3 was further evaluated by a standard HTLV-II serum panel established from the HTLV-I/II epidemiological surveillance of Colombian natives. Results: PROBLOT HTLV-I showed high R-values (greater than or equal to 0.980) for p28, p53 and gp46, but low R-values (< 0.900) for p19 and p24, Eitest ATL-WB showed a high R-value for pig, but low R-values for p53, gp46 and rgp21. HTLV BLOT 2.2 showed high R-values for p28 and recombinant gp46-I, but low R-values for p53 and gp46. The HTLV-II serum panel showed positive reactions with p24 and p19 of HTLV-I but a little if any reactions with p28 and gp46 of HTLV-I, Although these reactions could not define HTLV-II-specific WB patterns in anti-HTLV-I WB, HTLV-I- and HTLV-II-specific recombinant gp46 in HTLV BLOT 2.3 were useful to distinguish anti-HTLV-II antibodies. Conclusions: The currently available anti-HTLV-I WB kits are sufficient for confirmatory testing of anti-HTLV-I antibodies with WHO criteria using one gag and one env positivity, but they are inadequate for anti-HTLV-II confirmatory testing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:39:27