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Titolo:
BOTH ALLELIC FORMS OF THE RAT T-CELL DIFFERENTIATION MARKER RT6 DISPLAY NICOTINAMIDE ADENINE-DINUCLEOTIDE (NAD)-GLYCOHYDROLASE ACTIVITY, YET ONLY RT6.2 IS CAPABLE OF AUTOMODIFICATION UPON INCUBATION WITH NAD
Autore:
HAAG F; ANDRESEN V; KARSTEN S; KOCHNOLTE F; THIELE HG;
Indirizzi:
UNIV HAMBURG,HOSP EPPENDORF,MED KLIN,IMMUNOL ABT,MARTINISTR 52 D-20246 HAMBURG GERMANY UNIV HAMBURG,HOSP EPPENDORF,MED KLIN,IMMUNOL ABT D-20246 HAMBURG GERMANY
Titolo Testata:
European Journal of Immunology
fascicolo: 8, volume: 25, anno: 1995,
pagine: 2355 - 2361
SICI:
0014-2980(1995)25:8<2355:BAFOTR>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARGININE ADP-RIBOSYLTRANSFERASE; SKELETAL-MUSCLE CELLS; ESCHERICHIA-COLI; PERTUSSIS TOXIN; LYMPHOCYTES-T; SPLEEN-CELLS; S-1 SUBUNIT; BB/W RATS; RIBOSE; EXPRESSION;
Keywords:
RT6; ADP-RIBOSYLTRANSFERASE; NAD-GLYCOHYDROLASE; T CELL ECTOENZYMES; AUTOMODIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
F. Haag et al., "BOTH ALLELIC FORMS OF THE RAT T-CELL DIFFERENTIATION MARKER RT6 DISPLAY NICOTINAMIDE ADENINE-DINUCLEOTIDE (NAD)-GLYCOHYDROLASE ACTIVITY, YET ONLY RT6.2 IS CAPABLE OF AUTOMODIFICATION UPON INCUBATION WITH NAD", European Journal of Immunology, 25(8), 1995, pp. 2355-2361

Abstract

The finding that recently cloned mono-ADP-ribosyltransferases show sequence similarity to the rat T cell differentiation marker RT6 has ledus to investigate the enzymatic activity of this alloantigenic system. To search for ADP-ribosylation of cell surface proteins,T cell populations from RT6.1- and RT6.2-expressing rat strains, as well as RT6.1() and RT6.2(+) T-T hybridoma cell lines, were incubated with [P-32]nicotinamide adenine dinucleotide (NAD). All RT6.2(+), but no RT6.1(+) or RT6(-) cells, show incorporation of radioactivity into a single protein which could be identified as RT6.2 by immunoprecipitation with monoclonal antibodies. This automodification of RT6.2 is covalent, requires intact NAD as substrate, and displays characteristics typical for linkage of ADP-ribose to arginine. The alloantigens RT6.1 and RT6.2 differ in ten amino acids, RT6.2 having two arginine residues not presentin RT6.1. Both alloantigens were found to display potent NAD-glycohydrolase activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 17:48:46