Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ERYTHROCYTE STAGES OF PLASMODIUM-FALCIPARUM EXHIBIT A HIGH NITRIC-OXIDE SYNTHASE (NOS) ACTIVITY AND RELEASE AN NOS-INDUCING SOLUBLE FACTOR
Autore:
GHIGO D; TODDE R; GINSBURG H; COSTAMAGNA C; GAUTRET P; BUSSOLINO F; ULLIERS D; GIRIBALDI G; DEHARO E; GABRIELLI G; PESCARMONA G; BOSIA A;
Indirizzi:
UNIV TURIN,DIPARTIMENTO GENET BIOL & CHIM MED,VIA SANTENA 5 BIS I-10126 TURIN ITALY HEBREW UNIV JERUSALEM,INST LIFE SCI,DEPT BIOL CHEM IL-91904 JERUSALEMISRAEL MUSEUM NATL HIST NAT,BIOL PARASITAIRE & CHIMIOTERAPIE LAB F-75005 PARIS FRANCE UNIV TURIN,INST INTERNAL MED I-10100 TURIN ITALY
Titolo Testata:
The Journal of experimental medicine
fascicolo: 3, volume: 182, anno: 1995,
pagine: 677 - 688
SICI:
0022-1007(1995)182:3<677:ESOPEA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; VASCULAR ENDOTHELIAL-CELLS; L-ARGININE; CEREBRAL MALARIA; SIGNAL TRANSDUCTION; MURINE MACROPHAGES; INVIVO INDUCTION; POSSIBLE ROLES; CGMP LEVELS; MECHANISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
D. Ghigo et al., "ERYTHROCYTE STAGES OF PLASMODIUM-FALCIPARUM EXHIBIT A HIGH NITRIC-OXIDE SYNTHASE (NOS) ACTIVITY AND RELEASE AN NOS-INDUCING SOLUBLE FACTOR", The Journal of experimental medicine, 182(3), 1995, pp. 677-688

Abstract

Nitric oxide (NO), a highly diffusible cellular mediator involved in a wide range of biological effects, has been indicated as one of the cytotoxic agents released by leukocytes to counteract malaria infection. On the other hand, NO has been implicated as a mediator of the neuropathological symptoms of cerebral malaria. In such circumstances NO production has been thought to be induced in host tissues by host-derived cytokines. Here we provide evidence for the first time that human red blood cells infected by Plasmodium falciparum (IRBC) synthesize NO. The synthesis of NO (measured as citrulline and nitrate production) appeared to be very high in comparison with human endothelial cells; no citrulline and nitrate production was detectable in noninfected red blood cells. The NO synthase (NOS) activity was very high in the lysate of IRBC (while not measurable in that of normal red blood cells) and was inhibited in a dose-dependent way by three different NOS inhibitors(L-canavanine, N-G-amino-L-arginine, and N-G-nitro-L-arginine). NOS activity in P. falciparum IRBC is Ca++ independent, and the enzyme shows an apparent molecular mass <100 kD, suggesting that the parasite expresses an isoform different from those found in mammalian cells. IRBC release a soluble factor able to induce NOS in human endothelial cells. Such NOS-inducing activity is not tissue specific, is time and dose dependent, requires de novo protein synthesis, and is probably associated with a thermolabile protein having a molecular mass >100 kD. Our data suggest that an increased NO synthesis in P. falciparum malaria can be directly elicited by soluble factor(s) released by the blood stages of the parasite, without necessarily requiring the intervention of host cytokines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:29:57