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Titolo:
NATURAL-KILLER-CELL ACTIVATION-ASSOCIATED INDUCTION OF TARGET-CELL DNA FRAGMENTATION IN A SPONTANEOUSLY REGRESSING RAT HISTIOCYTOMA
Autore:
BRIGHT JJ; KAUSALYA S; KHAR A;
Indirizzi:
CTR CELLULAR & MOLEC BIOL HYDERABAD 500007 ANDHRA PRADESH INDIA CTR CELLULAR & MOLEC BIOL HYDERABAD 500007 ANDHRA PRADESH INDIA
Titolo Testata:
Immunology
fascicolo: 4, volume: 85, anno: 1995,
pagine: 638 - 644
SICI:
0019-2805(1995)85:4<638:NAIOTD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATORY FACTOR NKSF; TOXIC LYMPHOCYTES-T; LYMPHOKINE GENES; APOPTOSIS; INTERLEUKIN-2; RECEPTOR; DEATH; TRANSCRIPTION; ENDONUCLEASE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Bright et al., "NATURAL-KILLER-CELL ACTIVATION-ASSOCIATED INDUCTION OF TARGET-CELL DNA FRAGMENTATION IN A SPONTANEOUSLY REGRESSING RAT HISTIOCYTOMA", Immunology, 85(4), 1995, pp. 638-644

Abstract

The spontaneous regression of AK-5 histiocytoma is mediated by natural killer (NK) cells through antibody-dependent cell-mediated cellular cytotoxicity (ADCC) and the target cell death involves necrosis and apoptosis. We have studied the NK cell activation and the associated induction of apoptosis in the AK-5 tumour in rats. NK cells from immune animals expressed very low levels of CD16 and CD25 surface receptors, as revealed by Northern hybridization and flow cytometry. Interaction between NK cells and antibody-tagged AK-5 cells triggered the expression of these receptors to a higher level and affected AK-5 killing. Treatment of naive NK cells in vitro with interleukin-2 (IL-2), interferon-gamma (IFN-gamma) and IL-12 also enhanced the expression of these activation markers. Co-culture of NK cells from immune animals with antibody-tagged AK-5 cells induced formation of nuclear bodies in AK-5 and extensive fragmentation of AK-5 cell DNA. NK-mediated apoptosis was inhibited by zinc, actinomycin D and cycloheximide. The in vitro treatment of NK cells with cytokines enhanced their ability to induce apoptosis in AK-5 tumour. These results suggest that the NK cells acquire their ability to induce apoptosis in AK-5 tumour in association with their optimal activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 01:59:14