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Titolo:
CYCLOSPORINE STIMULATES EXPRESSION OF TRANSFORMING GROWTH-FACTOR-BETAIN RENAL-CELLS - POSSIBLE MECHANISM OF CYCLOSPORINES ANTIPROLIFERATIVE EFFECTS
Autore:
WOLF G; THAISS F; STAHL RAK;
Indirizzi:
UNIV HAMBURG,HOSP EPPENDORF,DEPT MED,DIV NEPHROL & OSTEOL,PAVIL 61,MARTINISTR 52 D-20246 HAMBURG GERMANY UNIV HAMBURG,DEPT MED,DIV NEPHROL & OSTEOL HAMBURG GERMANY
Titolo Testata:
Transplantation
fascicolo: 3, volume: 60, anno: 1995,
pagine: 237 - 241
SICI:
0041-1337(1995)60:3<237:CSEOTG>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROXIMAL TUBULAR CELLS; MESANGIAL CELLS; TGF-BETA; TUBULOINTERSTITIAL FIBROBLASTS; KIDNEY-DISEASE; CULTURE; NEPHROTOXICITY; TRANSCRIPTION; PROLIFERATION; HYPERTROPHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
G. Wolf et al., "CYCLOSPORINE STIMULATES EXPRESSION OF TRANSFORMING GROWTH-FACTOR-BETAIN RENAL-CELLS - POSSIBLE MECHANISM OF CYCLOSPORINES ANTIPROLIFERATIVE EFFECTS", Transplantation, 60(3), 1995, pp. 237-241

Abstract

CsA induces a reversible inhibition of proliferation in cultured murine proximal tubular cells (MCT cells) and syngeneic tubulointerstitialfibroblasts (TFB). To test whether this effect may be caused by endogenous synthesis and release of transforming growth factor-beta(1) (TGF-beta(1)), a well-known inhibitor of mitosis, MCT cells and TFB grown in serum-free media were treated with different concentrations of CsA. CsA, in a dose-dependent manner in a range of 500-2000 ng/ml, stimulated expression of TGF-beta(1) protein and steady-state mRNA levels in both cell lines (MCT cells: controls, 9.3+/-1.0; 1500 ng/ml CsA, 19.1+/-6.1 pg TGF-beta(1)/10(3) cells [P<0.05 vs. controls]; TFB: controls,5.4+/-0.9; 1500 ng/ml CsA, 7.7+/-0.3 pg TGF-beta(1)/10(3) cells; n=6). Short-term daily intraperitoneal injections of CsA (40 mg/kg body weight/day) into SJL mice for 1 and 4 weeks also induced an increase in whole kidney levels of TGF-beta(1) mRNA. Incubation of MCT cells and TFB with CsA in the presence of 30 mu g/ml of a neutralizing anti-TGF-beta(1-3) mAb partly reversed the cell cycle arrest induced by CsA. These data suggest that CsA-mediated intrarenal synthesis and release of TGF-beta(1) may play a role in the CsA-induced growth arrest and mighttherefore be relevant in the development of chronic CsA nephrotoxicity, which is characterized by striped fibrosis and tubular atrophy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 15:41:35